BLOOD CULTURE-VENIPUNCTURE

A positive bacteria result, depending on the organism isolated, may reflex additional identification and/or susceptibility charges

BL-VENI

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Adults Transfer 8 mL to 10 mL (minimum volume: 3 mL) of blood to a BACTEC Plus Aerobic/F culture bottle and 5 mL to 7 mL (minimum volume: 3 mL) of blood to a BACTEC Standard Anaerobic/F culture bottle. Infants/Children/Difficult to Draw Patients Transfer 1 mL to 3 mL (minimum volume: 0.5 mL) of blood to a BACTEC Peds P1us/F culture bottle. Recommended Volumes Adults: 13 mL to 17 mL per venipuncture Children: 1 mL to 5 mL per venipuncture Neonates: >1 mL per venipuncture Draw specimen as follows: 1. Pinch the wings of a ChloraPrep One-Step Frepp Applicator (2% chlorhexidine gluconate/70% isopropyl alcohol) to break its ampule and release the antiseptic. Thoroughly cleanse the puncture site by pressing the sponge against the skin and scrubbing in a back and forth motion for 30 seconds. 2. Allow 30 seconds for the venipuncture site to dry. 3. Do not palpate vein to be entered unless palpating finger is covered with a sterile glove or has been prepared in same way as puncture site. Note: If the patient is <2 months of age, cleanse the puncture site with 70% alcohol, followed by 30 seconds of scrubbing in a circular motion with tincture of iodine. Allow at least 2 minutes for the iodine to dry before venipuncture. Obtain blood and transfer to a BACTEC Peds Plus/F culture bottle and deliver immediately to laboratory. 

1 BLOOD CULTURE AEROBIC BOTTLE and 1 BLOOD CULTURE ANAEROBIC BOTTLE or 1 BLOOD CULTURE PEDS BOTTLE

No growth after 5 days