MYCOBACTERIAL CULTURE/SMEAR

A positive mycobacteria result will reflex additional identification charges. Additional susceptibility charges will also reflex if the isolate is identified as Mycobacterium tuberculosis complex

AFB

"MYCOBACTERIAL CULTURE
MYCOBACTERIAL SMEAR
"

See Transport Container for information

Submit only 1 of the following specimens: Bronchial Washing, Gastric Washing, or Tracheal Aspirate 1. Collect specimen in a 20-mL Luki sterile aspirating tube. Note: Specimen must be collected and received in sterile, sealed, leak proof container. 2. Maintain sterility and forward promptly. Note: 1.Specimen source is required on request form for processing. 2. One specimen/ 24 hour period/ site. 3. Up to 3 gastric aspirates/admission will be accepted. Sputum 1. Collect specimen in a screw-capped, sterile container. Note: Specimen must be collected and received in sterile, sealed, leak proof container. 2. Preferred specimen is a single, first-morning, ""deep cough"" sputum specimen; and the patient should not have eaten prior to collection. When antibiotics are to be started immediately, a first-morning specimen is still preferred. 3. Three specimens should be collected over an 8 to 24 hour period and should include at least 1 first-morning specimen. 4. Instruct patient to brush his/her teeth and/or rinse mouth well with water to remove any indigenous flora from the mouth area. 5. Have patient remove dentures. 6. Instruct patient to take a deep breath, hold it momentarily, and cough deeply and vigorously into container. Close lid securely. 7. Maintain sterility and forward promptly. If there is a delay in transport of >1 hour, send specimen refrigerated. Note: 1. Cough induction by inhalation of a saline aerosol may be used if required. 2. Specimen source is required on request form for processing. 3. Up to 5 sputum specimens/admission will be accepted. Stool Stool specimens are recommended only for detection of Mycobacterium avium-complex involvement in gastrointestinal tracts of patients with acquired immunodeficiency syndrome. 1. A minimum of 1 g of stool in a screw-capped, sterile container. Note: Specimen must be collected and received in sterile, sealed, leak proof container. 2. Maintain sterility and forward promptly. Note: Specimen source is required on request form for processing. Tissue 1. Tissue specimen in a screw-capped, sterile container. Note: Specimen must be collected and received in sterile, sealed, leak proof container. 2. Maintain sterility and forward promptly. Note: Specimen source is required on request form for processing. Urine (Midstream Clean-Catch) 1. First-morning specimen is preferred. 2. Collect entire urine specimen in a screw-capped, sterile container. (Timed urine specimen is not acceptable.) Note: Specimen must be collected and received in sterile, sealed, leak proof container. 3. Maintain sterility and forward promptly. Note: 1. Follow instructions in ""Midstream Clean-Catch Urine Collection"" in ""Urine Collection"" in ""Special Instructions."" 2. Specimen source is required on request form for processing. 3. Up to 5 urine specimens/admission will be accepted. 

1 STERILE CONTAINER 

No growth after 8 weeks