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Factor VIII Antigen
MessagePlatelet poor plasma: Centrifuge light blue-top tube for 15
minutes at approximately 1500 g within 60 minutes of collection.
Using a plastic pipette, remove plasma, taking care to avoid the
WBC/platelet buffy layer and place into a
plastic screw-cap vial. Centrifuge a second time and transfer
platelet-poor plasma into a new plastic screw-cap vial. Plasma must
be free of platelets (<10, 000/mcL). Freeze immediately and ship
on dry ice. Do not thaw.
Test Code
F8G
Alias/See Also
LAB9290
Factor 8 antigen
CPT Codes
85244
Preferred Specimen
1 mL frozen platelet-poor plasma
Minimum Volume
0.5 mL
Instructions
Platelet poor plasma: Centrifuge light blue-top tube for 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic screw-cap vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic screw-cap vial. Plasma must be free of platelets (<10, 000/mcL). Freeze immediately and ship on dry ice. Do not thaw.
Transport Container
Plastic screw-cap vial
Transport Temperature
Frozen
Specimen Stability
Plasma
Room temperature: 4 hours
Refrigerated: 48 hours
Frozen (-20C): 30 days Frozen (-70C): 6 months
Methodology
Immunoassay (IA)
Setup Schedule
As needed: at least 1x/month
Report Available
2-31 days
Reference Range
0.60-1.95 IU/mL
Clinical Significance
An ELISA for Factor VIII antigen may be used in conjunction with the functional assays in the assessment of Factor VIII replacement therapies, assessment of carrier status, as well as to distinguish those patients that may be deemed cross-reactive material (CRM) positive.
Performing Laboratory
Quest Diagnostics Nichols Inst San Juan Capistrano 33608 Ortega Highway San Juan Capistrano, CA 92675
Last Updated: February 20, 2023