QuestAssureD™ 25-Hydroxyvitamin D (D 2, D 3)

Test Code

QD2D3

Alias/See Also

LAB9508

CPT Codes
<p>82306</p>

Includes

Vitamin D, 25-OH, Total
Vitamin D, 25-OH, D₃Vitamin D, 25-OH, D₂

Preferred Specimen

Patient Preparation: Fasting preferred, but not required

Specimen Type: Serum

Collection Container/Type

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

Collect blood in a Serum Separator Tube (SST®).
Allow to clot at room temperature.
Centrifuge and remove from the gel within 48 hours.
Alternatively, collect blood in a standard red-top serum Vacutainer® tube. Allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.

Patient Preparation
Fasting preferred, but not required

Minimum Volume

0.3 mL

Instructions

Collect blood in a standard red-top serum Vacutainer® tube. Allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.
Alternatively, collect blood in a serum separator tube (SST), allow to clot at room temperature. Centrifuge and remove from the gel within 48 hours.

Transport Container

Transport tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 21 days

Refrigerated: 21 days

Frozen: 21 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis • Gross lipemia • Heparinized or EDTA plasma • Serum not separated from Serum Separator Tube (SST®) gel or clot within 48 hours • Grossly icteric

Methodology

Chromatography/Mass Spectrometry

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Mon-Sat; Report available: 2 days

Report Available

2 days

Reference Range

Vitamin D, 25-OH, Total	30-100 ng/mL
Vitamin D, 25-OH, D3	Not established
Vitamin D, 25-OH, D2	Not established

Clinical Significance

Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead to hypercalcemia. This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-hydroxy vitamin D: 25-OH D₃ - the endogenous form of the vitamin and 25-OH D₂ - the analog form used to treat 25-OH Vitamin D₃ deficiency.

Performing Laboratory
Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Last Updated: February 17, 2023

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.