MPL Mutation Analysis

Test Code

MPLMA

Alias/See Also

LAB14055

CPT Codes
<p>81339</p>

Preferred Specimen

Submit only 1 of the following specimens:

Specimen Type: Peripheral blood

Collection Container/Type

Preferred: Lavender top (EDTA)

Acceptable: Green top (Sodium heparin)

Specimen Volume: 4 mL

Specimen Type: Bone marrow

Collection Container/Type

Preferred: Lavender top (EDTA)

Acceptable: Green top (Sodium heparin)

Specimen Volume: 3 mL

Other specimen types (fixed cell pellets, extracted DNA, etc.) acceptable. See Quest Diagnostics testing directory for instructions on other specimen types not listed.

Minimum Volume

3 mL whole blood

1 mL bone marrow aspirate

Other Acceptable Specimens

Whole blood or bone marrow aspirate collected in a sodium heparin (green-top) tube • Fixed Cell pellet collected in a plastic leak-proof container • Extracted DNA from CLIA-certified laboratory collected in a sterile leak-proof container

Instructions

Note: Do not reject specimens, send to lab for screening.

Cell Pellets: Send fixed samples within 7 days of fixation date in a plastic, leak-proof tube/container. Indicate type of fixative used along with patient name, specimen source, date collected, and date harvested/fixed. Cell pellets submitted in Carnoy's or other alcohol-based fixatives are acceptable.

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 7 days

Refrigerated: 7 days

Frozen: Unacceptable

Methodology

Polymerase Chain Reaction-based DNA Sequencing

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Daily; Report available: 5-7 days

Report Available

5-7 days

Reference Range

See Laboratory Report

Clinical Significance

This DNA-based assay tests leukocytes from blood or bone marrow aspirate for mutations in exon 10 of the thrombopoietin receptor (MPL), including codons 505 and 515, using an advanced DNA sequencing method. Mutations at these sites in MPL are associated with myeloproliferative neoplasms, particularly essential thrombocythemia and primary myelofibrosis.

Performing Laboratory
Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Last Updated: February 17, 2023

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.