T-Spot. TB

Test Code

TSPTQ

Alias/See Also

LAB13087

CPT Codes
86481<br>Restricted Client Code

Preferred Specimen

Collection Conatiner/Tube

Preferred: Green Lithium Heparin, Whole Blood

Acceptable: Green Sodium Heparin, Whole Blood

Submission Container/Tube: Green Lithium
Heparin tube or Green Sodium Heparin tube

Specimen Volume: 9 mL

Collection Instructions: Collect blood by
venipuncture and invert (8-10 times) to ensure that the whole blood
is mixed thouroughly with the anticoagulant.

Minimum Volume

9.0 mL

Instructions

Collect blood by venipuncture into a Quest-provided Greiner 9 mL lithium heparin (green-top) tube. The tube contents must be inverted (8-10 times) to ensure that the whole blood is mixed thoroughly with the anticoagulant. Specimens must be shipped in accordance to the pre-approved logistics process established for your site. Samples received >39 hours after collection will be rejected. Date and time of draw are required and must be provided.

Note: *****This is a short stability test and requires special handling. Please see above for special collection instructions. If you have any questions, please contact Customer Service 1-800-336-3718 option 1.*****

Transport Container

See instructions

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 39 hours

Refrigerated: Unaccpetable

Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Anticougulant other than lithium heparin or
sodium heparin hemolized: Centtrifuged, Samples
received >39 hours after collection. Frozen or
refrigerated samples.

Methodology
Enzyme-Linked Immunosorbent Assay

Setup Schedule

Sunday - Saturday

Report Available

Next day

Reference Range

See Laboratory Report

Clinical Significance

The T-SPOT®.TB test is an in vitro diagnostic test for the detection of effector T cells that respond to stimulation by Mycobacterium tuberculosis antigens ESAT-6 and CFP 10 by capturing interferon gamma (IFN-y) in the vicinity of T cells in human whole blood collected in lithium heparin. It is intended for use as an aid in the diagnosis of M. tuberculosis infection. The T-SPOT.TB test is an indirect test for M. tuberculosis infection (including disease) and is intended for use in conjunction with risk assessment, radiography and other medical and diagnostic evaluations.

Performing Laboratory
Quest Diagnostics Nichols Institute 14225 Newbrook Drive Chantilly, VA 20153

Last Updated: February 17, 2023

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.