A B C D E F G H I J K L M N O P Q R S T U V W X Y Z #

Bordetella pertussis/parapertussis DNA, Qualitative, Real-Time PCR

Test Code
BORPC


Alias/See Also

LAB9342



CPT Codes
<p>87798 (x2)</p>

Includes
Bordetella pertussis DNA and Bordetella parapertussis DNA


Preferred Specimen

Submit only 1 of the following specimens:

 

Specimen Type: Nasopharyngeal (not throat) aspirate/wash or nasal aspirate/wash

Collection Container/Type: Sterile container (no transport media)

Submission Container/Tube: Sterile container

Specimen Volume: Entire collection

Collection Instructions: 

  1. Instill 1-1.5 mL of nonbacteriostatic saline (pH 7.0) into one nostril.
  2. Flush a plastic catheter or tubing with 2-3 mL of saline. I
  3. nsert the tubing into the nostril parallel to the palate.
  4. Aspirate nasopharyngeal secretions.
  5. Repeat this procedure for the other nostril.
  6. Combine aspirates into a sterile vial.

 

Specimen Type: Nasopharyngeal swab

Collection Container/Type

Preferred: Liquid Aimes

Acceptable: VCM or equivalent UTM, M4, or eSwab (Liquid Aimes)

Submission Container/Tube: Liquid Amies

Specimen Volume: Swab

Collection Instructions: 

  1. Collection kits using Amies liquid, M4, VCM/UTM are acceptable.
  2. Swab tips may be polyester, rayon, nylon flocked.
  3. Flexible shafts must be used for proper collection.
  4. Accuracy of the test depends on the collection of ciliated epithelial cells material.
  5. Half the distance from the nostril to the base of the ear could be used as estimation of the site of collection.
  6. Gently insert swab into the nostril. Keep the swab near the septum floor of the nose while gently pushing into the posterior nasopharynx.
  7. Rotate swab 2-3 times and hold in place for few seconds for optimal collection.
  8. Collection of both nostrils is recommended but not required.


Minimum Volume

Entire collection



Other Acceptable Specimens
Nasopharyngeal swab submitted in a V-C-M medium (green-cap) tube, or equivalent UTM or multi-microbe media (M4), or eSwabs (Liquid Amies)


Instructions
Nasopharyngeal aspirates:
Instill 1-1.5 mL of nonbacteriostatic saline (pH 7.0) into one nostril. Flush a plastic catheter or tubing with 2-3 mL of saline. Insert the tubing into the nostril parallel to the palate. Aspirate nasopharyngeal secretions. Repeat this procedure for the other nostril. Combine aspirates into a sterile vial.

Nasopharyngeal swab:
Collection kits using Amies liquid, M4, VCM/UTM are acceptable. Swab tips may be polyester, rayon, nylon flocked. Flexible shafts must be used for proper collection. Accuracy of the test depends on the collection of ciliated epithelial cells material. Half the distance from the nostril to the base of the ear could be used as estimation of the site of collection. Gently insert swab into the nostril. Keep the swab near the septum floor of the nose while gently pushing into the posterior nasopharynx. Rotate swab 2-3 times and hold in place for few seconds for optimal collection. Collection of both nostrils is recommended but not required.


Transport Temperature
Refrigerated (cold packs)


Specimen Stability

Room temperature: 48 hours


Refrigerated: 7 days


Frozen: 30 days



Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Collection tubes with Amies gel or charcoal containing media • Samples collected using cotton or calcium alginate tipped swabs • Rigid shaft swabs



Methodology

Real-Time Polymerase Chain Reaction (PCR)

This test detects but does not distinguish between B. Pertussis and B. Holmesii.

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.



FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Set up: Daily; Report available: 1-2 days


Report Available

1-2 days



Reference Range
B. pertussis DNA Not detected
B. parapertussis DNA Not detected


Clinical Significance
Bordetella pertussis is the cause of whooping cough that may occur in unimmunized individuals. B. parapertussis is a related organism that causes a similar, but milder, disease. Laboratory diagnosis may require both culture and serological confirmation, although culture is difficult.


Performing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153




Last Updated: February 17, 2023


The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.