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BK Virus DNA, Quantitative Real-Time PCR, Plasma

Message
Effective 06/08/2024: ​Test is being replaced by BKVG / LAB17071 Plasma, BK Virus DNA, Quantitative RT PCR performed at Inova Laboratories. 


Test Code
BK


Alias/See Also

Epic: LAB1374




CPT Codes
<p>87799</p>

Preferred Specimen

Submit only 1 of the following specimens:

 

Specimen Type: Whole Blood

Collection Container/Type

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD A or B)

Specimen Volume: 0.7 mL

Collection Instructions: 

  • Collect using standard procedures. Store samples refrigerated following collection. Do not freeze whole blood.

 

Specimen Type: Plasma EDTA

Collection Container/Type: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 0.7 mL

Collection Instructions: 

  • Collect blood in sterile tubes containing EDTA as an anticoagulant, or in Plasma Preparation Tube (PPT). Collect whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw-cap aliquot tubes. Freeze and ship frozen.

 

Specimen Type: Serum

Collection Container/Type: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 0.7 m:

Collection Instructions: 

  • Collect blood in sterile tubes with no anticoagulants; Serum Separator Tubes (SST ®s) are recommended. Allow blood to clot at room temperature and separate serum within 1 hour of collection. Transfer serum to sterile, plastic, screw-cap aliquot tubes. Freeze and ship frozen. 


Minimum Volume

0.3 mL



Instructions
Plasma: Collect blood in sterile tubes containing EDTA as an anticoagulant, or in plasma preparation tube (PPT). Collect whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, transport aliquot tubes. Freeze and ship frozen.

Serum: Collect blood in sterile tubes with no anticoagulants; serum separator tubes (SST) are recommended. Allow blood to clot at room temperature and separate serum within 1 hour of collection. Transfer serum to sterile, transport aliquot tubes. Freeze and ship frozen.

Whole blood: Collect using standard procedures. Store samples refrigerated following collection. Do not freeze whole blood.


Transport Temperature
Plasma or serum: Frozen
Whole blood: Refrigerated (cold packs)


Specimen Stability

Whole Blood


Room temperature: 48 hours


Refrigerated: 8 days


Frozen: Unacceptable


 


Plasma or Serum


Room temperature: 48 hours


Refrigerated: 7 days


Frozen: 30 days



Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Samples collected in heparin • Unspun PPT tube • Frozen whole blood • Serum collected in a no additive (red-top) tube



Methodology

Real-Time Polymerase Chain Reaction (RT-PCR) 

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
 



FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Set up: Daily; Report available: 1 day


Report Available

1 day



Reference Range
BK Virus DNA,QN RT PCRNot Detected (copies/mL)
BK Virus DNA,QN RT PCRNot Detected (Log copies/mL)


Clinical Significance
This test is used to determine the presence of BK Virus DNA in patient's specimens. Detection of the virus in these specimens may be indicative of an active infection, as PCR detects the presence of the virus, and not the host's reaction to the virus. Polyomavirus BK DNA detection in urine or plasma is associated with an increased risk of graft rejection in renal recipients. Quantitative testing may indicate change in risk over time. This is a quantitative molecular test, with a linear range of 500-500,000,000 copies/mL.


Performing Laboratory

Quest Diagnostics Nichols Institute 
14225 Newbrook Drive 
Chantilly, VA 20153




Last Updated: April 4, 2024


The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.