A B C D E F G H I J K L M N O P Q R S T U V W X Y Z #

5-Hydroxyindoleacetic Acid (5-HIAA), 24-Hour Urine without Creatinine

Test Code
5HIAB


Alias/See Also

LAB2910



CPT Codes
<p>83497</p>

Includes
CPT code 81050 may be added at an additional charge for volume measurement


Preferred Specimen

Patient Preparation: Patient should avoid food high in indoles: avocado, banana, tomato, plum, walnut, pineapple, and eggplant.

Specimen Type: Urine

Collection Container/Type

Preferred: 24-hour urine (preserved: 25 mL 6N HCl)

Acceptable: 24-hour urine (unpreserved with a pH ≤6)

Submission Container/Tube: Sterile container

Specimen Volume: 10 mL

Collection Instructions: 

  1. Collect 24-hour urine with 25 mL 6N HCl to maintain a pH ≤3.  
  2. If no preservative is used, specimen must be stored refrigerated during collection but shipped frozen and pH must be ≤6.
  3. Record 24-hour urine volume and patients age on test request form and urine container.

 

24-Hour Patient Urine Collection Instructions



Patient Preparation
Patient should avoid food high in indoles: avocado, banana, tomato, plum, walnut, pineapple, and eggplant

Minimum Volume

5 mL



Other Acceptable Specimens
Aliquot from a 24-hour urine, unpreserved with a pH ≤6, collected in a plastic 24-hour urine container


Instructions

Collect 24-hour urine with 25 mL 6N HCl to maintain a pH ≤3.  If no preservative is used, specimen must be stored refrigerated during collection but shipped frozen and pH must be ≤6.
Record 24-hour urine volume and patient's age on test request form and urine container.



Transport Temperature
Preserved urine: Room temperature
Unpreserved urine: Frozen


Specimen Stability

Preserved urine


Room temperature: 7 days


Refrigerated: 30 days


Frozen: 30 days


 


Unpreserved urine


Room temperature: Unacceptable


Refrigerated: Unacceptable


Frozen: 30 days



Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Preserved urine received refrigerated with a pH >3 • Unpreserved urine received frozen with pH >6



Methodology

High Performance Liquid Chromatography (HPLC)

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.



FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Tuesday-Saturday Morning Report available: Next Day


Report Available

1-3 days



Limitations

Patients with renal disease may display falsely decreased results. Urinary 5-HIAA is increased in patients with malabsorption disorders who also display a larger concentration of urinary tryptophan metabolites. Urinary 5-HIAA is increased in patients with intestinal obstruction and with some noncarcinoid islet cell tumors. Carcinoid syndrome may not cause elevated results, especially if the patient does not have diarrhea.



Reference Range
<2 YearsNot established
2-10 Years≤8.0 mg/24 h
>10 Years≤6.0 mg/24 h


Clinical Significance
Values above 10 mg/24-hours occur in carcinoid syndrome. Slight elevations also occur in nontropical sprue and after reserpine administration. Drugs which can interfere with testing due to physiologic response: Acetaminophen, alpha and beta blockers, atenolol, bromocriptine, broncholdilators, clonidine, digoxin, isoniazid, L-dopa, labetalol, methyldopa, monoamine oxidase inhibitors, nitroglycerin, sympathomimetic amines, phenobarbital, phenothiazines, phentolamine, reserpine, salicylates, tricyclic antidepressants.


Performing Laboratory

Quest Diagnostics Nichols Inst San Juan Capistrano
33608 Ortega Highway
San Juan Capistrano, CA 92690-6130




Last Updated: February 17, 2023


The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.