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Lyme Disease, Molecular Detection, PCR, Varies
MessageTest Code
Preferred Specimen
Ordering Guidance
This assay does not detect Borrelia miyamotoi. If infection with this organism is suspected, order BMIYB / Borrelia miyamotoi Detection PCR, Blood or BMIYC / Borrelia miyamotoi Detection PCR, Spinal Fluid.
Necessary Information
Specimen source is required.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Spinal fluid
Container/Tube: Sterile vial
Specimen Volume: 1 mL
Collection Instructions: Label specimen as spinal fluid.
Specimen Type: Synovial fluid
Container/Tube: Sterile vial
Specimen Volume: 1 mL
Collection Instructions: Label specimen as synovial fluid.
Specimen Type: Tissue (fresh only)
Sources: Skin or synovial biopsy
Container/Tube: Sterile container with normal saline
Specimen Volume: Approximately 4 mm(3)
Collection Instructions:
1. Submit only fresh tissue.
2. Skin biopsies:
a. Wash biopsy site with an antiseptic soap. Thoroughly rinse area with sterile water. Do not use alcohol or iodine preparations. A local anesthetic may be used.
b. Biopsy specimens are best taken by punch biopsy to include full thickness of dermis.
3. Label specimen with source of tissue.
Minimum Volume
Spinal Fluid, Synovial Fluid: 0.3 mL
Tissue: NA
Methodology
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Setup Schedule
June through November: Monday through Saturday
December through May: Monday through Friday
Report Available
Reference Range
Negative
Performing Laboratory
Mayo Clinic Laboratories 200 First Street SW Rochester, MN 55905
Last Updated: February 16, 2023