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Testosterone, Total
Test CodeAlias/See Also
Preferred Specimen
Specimen Type: Serum
Collection Container: Serum gel
Specimen Volume: 3 mL
Minimum Volume
0.10 mL
Other Acceptable Specimens
Specimen Type: Plasma
Collection Container: Green top (Lithium heparin)
Instructions
Transport Container
Specimen Stability
Room temperature: 8 hours
Refrigerated (on gel): 8 hours
Refrigerated (off gel): 7 days
Frozen: 7 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Samples stored on gel >8 hours, unlabeled, mislabeled, wrong tube type, hemolyzed, QNS, exceeds specimen stability requirements.
Methodology
Chemiluminescent Microparticle Immunoassay (CMIA)
Setup Schedule
Daily
Report Available
Same day
Limitations
If the Testosterone results are inconsistent with clinical evidence, additional testing is suggested to confirm the result.
Specimens from patients who have received preparations of mouse monoclonal antibodies for diagnosis or therapy may contain human anti-mouse antibodies (HAMA). Such specimens may show either falsely elevated or depressed values when tested with assay kits that employ mouse monoclonal antibodies. Heterophilic antibodies in human serum can react with reagent immunoglobulins, interfering with in vitro immunoassays. Patients routinely exposed to animals or to animal serum products can be prone to this interference and anomalous values may be observed. Additional information may be required for diagnosis.
Reference Range
Females (adult): 14 - 76 ng/dL
Clinical Significance
The 2nd Generation Testosterone assay releases testosterone from binding proteins and measures total testosterone. Free testosterone can be calculated from the total testosterone, sex hormone-binding globulin (SHBG) and albumin concentrations.
The concentration of testosterone fluctuates over 24 hours. The pulsatile release of LH in the night typically leads to a peak of testosterone concentration in the morning. Time of day, age, sex, puberty, pre- and post-menopause, and disease, all have an influence on testosterone concentration and should be considered in interpreting individual results.
Performing Laboratory
Inova Laboratories
2832 Juniper Street
Fairfax, VA 22031
Last Updated: April 12, 2023
Last Review: N. Wolford, March 9, 2023