A B C D E F G H I J K L M N O P Q R S T U V W X Y Z #

Mycoplasma pneumoniae DNA, Qualitative Real-Time PCR

Test Code
MPNED


Alias/See Also

LAB261



CPT Codes
<p>87581</p>

Preferred Specimen

Submit only 1 of the following specimens:

Specimen Type: Respiratory

Sources: Bronchial washing, bronchoalveolar lavage, sputum

Collection Container/Type: Sterile container

Submission Container/Tube: Sterile container

Specimen Volume: 1 mL

Collection Instructions: 

  • Sputum: Collect in a sputum collection kit or a sterile, leak-proof plastic container.
  • Bronchial Lavage: Collect in a sterile, leak-proof plastic container.

 

Specimen Type: Swab

Sources: Throat, nasal, or nasopharyngeal

Collection Container/Type

Preferred: VCM (green-cap)

Acceptable: M4-RT, Universal transport media (UTM), or equivalent

Specimen Volume: Swab

Collection Instructions: 

  • Nasopharyngeal and Throat swab in M4, VCM (UTM) Media (Use only sterile Dacron or rayon swabs.)
  • Do NOT use calcium alginate swabs, as they may contain substances that inhibit PCR testing.
  • Break applicator sticks off near the tip to permit tightening of the cap.

 

Specimen Type: Fluid

Sources: Cerebrospinal

Collection Container/Type: Sterile container

Submission Container/Tube: Sterile container

Specimen Volume: 1 mL



Minimum Volume

0.35 mL



Other Acceptable Specimens
1 mL CSF collected in a sterile leak-proof container


Instructions
Nasopharyngeal and Throat swab in M4, VCM (UTM) Media (Use only sterile Dacron or rayon swabs.) Do NOT use calcium alginate swabs, as they may contain substances that inhibit PCR testing. Break applicator sticks off near the tip to permit tightening of the cap.

Sputum: Collect in a sputum collection kit or a sterile, leak-proof plastic container.
Bronchial Lavage: Collect in a sterile, leak-proof plastic container.


Transport Temperature
Refrigerated (cold packs)


Specimen Stability

Room temperature: 48 hours

Refrigerated: 7 days

Frozen: 30 days



Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Calcium alginate swabs



Methodology

Real-Time Polymerase Chain Reaction

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.



FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Set up: Mon-Sat; Report available: 1-2 days


Report Available

1-2 days



Reference Range
Not detected


Clinical Significance
Mycoplasma pneumoniae DNA, PCR, is a highly sensitive method to detect the presence of Mycoplasma pneumoniae DNA in clinical specimens.
The diagnosis of Mycoplasma pneumoniae infection should not rely solely upon the result of a PCR assay. A positive result should be determined in conjunction with clinical presentation and additional established clinical tests. Moreover, this assay cannot differentiate between viable and nonviable organisms. A negative PCR result indicates the absence of Mycoplasma pneumoniae DNA at detectable levels in the sample tested and does not exclude the diagnosis of disease.


Performing Laboratory

Quest Diagnostics Nichols Institute-San Juan Capistrano, CA
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042




Last Updated: February 23, 2012


The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.