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Amikacin, Random
Test CodeAMIKR
Alias/See Also
Epic: LAB2930
Preferred Specimen
Specimen Type: Serum
Collection Container: Serum gel
Specimen Volume: 3 mL
Minimum Volume
0.5 mL
Other Acceptable Specimens
Collection Container: Red top
Instructions
- Serum gel tubes should be centrifuged within 4 hours of collection.
- Red top tubes should be centrifuged, and the serum aliquoted into a plastic vial within 4 hours of collection.
Transport Container
Plastic vial
Specimen Stability
Room temperature: Unaccpetable
Refrigerated: 7 days
Frozen: 14 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Unlabeled, mislabeled, wrong tube type, hemolyzed, visually lipemic, QNS, exceeds specimen/stability requirements.
Methodology
Particle-enhanced turbidimetric inhibition immunoassay (PETINIA)
Setup Schedule
Daily
Report Available
Same day
Limitations
Heterophile antibodies interfere, causing falsely elevated results. Patients receiving amikacin or kanamycin may yield falsely elevated values for tobramycin. Unless frozen, assay tobramycin samples containing carbenicillin or piperacillin within 8 hours to prevent falsely lowered tobramycin results. High concentrations of penicillins or cephalosporins have been shown to inactivate tobramycin in vitro.
Clinical Significance
Amikacin is a semi-synthetic aminoglycoside that exhibits bactericidal activity against a wide range of pathogens, including many organisms resistant to other aminoglycosides. Amikacin is active in vitro against gram-negative organisms, penicillinase, and non-penicillinase producing staphylococci. The strength of this drug is due primarily to its high degree of resistance to aminoglycoside inactivating enzymes. Determination of serum or plasma drug levels is required to achieve optimum therapeutic efficacy and minimize toxicity.
Performing Laboratory
Inova Laboratories
2832 Juniper Street
Fairfax, VA 22031
Last Updated: November 22, 2023
Last Review: N. Wolford, November 22, 2023