Protein Electrophoresis and Kappa/Lambda Light Chains, Serum

Test Code

LAB5211931

Quest Code

17099

CPT Codes
83883 (x2), 84155, 84165

Includes

Protein, Total and Protein Electrophoresis
Kappa/Lambda Light Chains, Total, Serum

Preferred Specimen

5 mL serum

Patient Preparation
Overnight fasting is preferred

Minimum Volume

1.6 mL

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 28 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis • Grossly lipemic • Plasma

Methodology
Capillary Zone Electrophoresis • Spectrophotometry (SP) • Nephelometry

Setup Schedule

Night Sets up 6 days a week.

Reference Range

Protein, Total

	Male (g/dL)	Female (g/dL)
<1 Month	4.1-6.3	4.2-6.2
1-5 Months	4.7-6.7	4.4-6.6
6-11 Months	5.5-7.0	5.6-7.9
1-19 Years	6.3-8.2	6.3-8.2
≥20 Years	6.1-8.1	6.1-8.1

Protein Electrophoresis

Albumin	3.8-4.8 g/dL
Alpha-1 Globulin	0.2-0.3 g/dL
Alpha-2 Globulin	0.5-0.9 g/dL
Beta-1 Globulin	0.4-0.6 g/dL
Beta-2 Globulin	0.2-0.5 g/dL
Gamma Globulin	0.8-1.7 g/dL
Abnormal Protein Band 1	None detected
Abnormal Protein Band 2	None detected
Abnormal Protein Band 3	None detected

Kappa/Lambda Light Chains, Total

Kappa	176-443 mg/dL
Lambda	91-240 mg/dL
Kappa/Lambda Ratio	1.29-2.55

Clinical Significance

Serum protein electrophoresis (SPE) is an analytical technique that provides separation of serum protein into six fractions: Albumin, Alpha-1, Alpha-2, Beta-1, Beta-2, and Gamma. Interpretation of elevation, decreased, or visual change in different fractions can be used as a diagnostic aid for a variety of different disease states and protein abnormalities, including monoclonal gammopathies (MG).

Performing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano, CA 92690-6130

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.