Varicella Zoster Virus Culture, Rapid Method

LAB5210017

2691

3 mL vesicle/lesion swab, throat swab or tissue biopsy in VCM tube or FDA approved equivalent viral transport media

1 mL

Eye swab or CSF in V-C-M medium (green-cap) tube or equivalent • Tracheal aspirate, bronchial lavage/wash, sputum, tissue biopsy, fresh (unfixed) tissue or CSF in sterile screw-cap container

To maintain optimum viability, place swab or fluid into VCM tube (equal volumes of fluid and media) or FDA approved equivalent viral transport media and transport the specimen to the laboratory as soon as possible. Best recovery is obtained when the specimens are refrigerated at 2-8° C or kept on wet ice following collection and while in transit.
If there will be a long delay before processing, specimens in transport media should be frozen at -70° C or colder and transported on dry ice. Storage or transport at -20° C is not acceptable. Raw (unpreserved) specimens should only be refrigerated and not frozen.

Note: Use Dacron® or rayon-tipped plastic or metal shaft swabs for specimen collection. Do not use wooden shaft or calcium alginate swabs as they may inhibit recovery of viruses and Chlamydia.

Refrigerated (cold packs)

Wooden shaft and calcium alginate swabs • Raw (unpreserved) stool specimens • Dry swabs • Transwabs® or gel-based transport medium • DNA probe transports • Tissues in formalin or other fixatives • Specimens on glass slides • Received room temperature • Specimen in V-C-M received frozen (-20° C) • CSF (sterile screw-cap container) received frozen • Unpreserved Samples received Room temperature or frozen

A.M.

Not isolated

Varicella-Zoster Virus (VZV) is a member of the herpes virus group that causes varicella (chicken pox) and zoster (shingles). Varicella is the primary infection while zoster is caused by reactivation of the latent virus. HSV and VZV infection may be difficult to distinguish clinically. VZV can be recovered from vesicle fluid usually during the first three days of rash. The incubation period of VZV is 14-15 days and clinical signs of varicella include fever and malaise followed by a rash rapidly progressing to macules to papules to vesicles to crusts over the trunk, neck, face, arms and legs. Zoster is characterized by an inflammatory reaction of the posterior nerve root and ganglia accompanied by crops of vesicles over the skin.