Sirolimus Level

Message

Sendout, Mayo test code: SIIRO

Test Code

LAB875

Alias/See Also

Rapamune
Rapamycin
SIIRO

CPT Codes
80195

Preferred Specimen

3 mL whole blood from lavender EDTA tube

Minimum Volume

1 mL

Other Acceptable Specimens

EDTA

Instructions

1. Draw blood immediately before a scheduled dose.
2. Do not centrifuge.
3. Send whole blood specimen in original tube. Do not aliquot.

Transport Container

Original tube

Transport Temperature

Refrigerated

Specimen Stability

Refrigerated (preferred): 28 days
Ambient: 28 days
Frozen: 28 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis: OK
Gross lipemia: OK
Gross icterus: OK

Methodology
High-Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS)

FDA Status
Approved

Setup Schedule

Monday through Sunday

Report Available

1-3 days

Limitations

The recommended therapeutic range applies to trough specimens collected immediately before a dose. Blood drawn at other times will yield higher results.

Reference Range

Included with report

Clinical Significance

Monitoring whole blood sirolimus concentration during therapy, particularly in individuals coadministered cytochrome P450 (CYP) 3A4 substrates, inhibitors, or inducers

Adjusting dose to optimize immunosuppression while minimizing toxicity

Evaluating patient compliance

Performing Laboratory
Mayo Clinic Laboratories, Rochester, Minnesota

Additional Information
Sirolimus, Whole Blood

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.