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Lactate Dehydrogenase, Isoenzyme
MessageSendout, Mayo test code: LD_I
Test Code
LAB97
Alias/See Also
Isoenzymes of Lactate Dehydrogenase (LD)
Lactate Dehydrogenase Isoenzymes
S LD (Lactate Dehydrogenase)
LD Isoenzymes LDH (Lactate Dehydrogenase)
LDH Fractionation LDH Isoenzymes
LD_I
Lactate Dehydrogenase Isoenzymes
S LD (Lactate Dehydrogenase)
LD Isoenzymes LDH (Lactate Dehydrogenase)
LDH Fractionation LDH Isoenzymes
LD_I
CPT Codes
83615, 83625
Preferred Specimen
2 mL serum in a gold serum gel tube
Minimum Volume
0.75 mL
Other Acceptable Specimens
Red tube
Instructions
Ambient
Transport Container
Plastic vial
Transport Temperature
Ambient
Specimen Stability
Ambient (preferred): 7 days
Refrigerated: 48 hours
Refrigerated: 48 hours
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis: Mild reject; Gross reject
Lipemia: Mild OK; Gross OK
Lipemia: Mild OK; Gross OK
Methodology
LDI: Electrophoresis Densitometry
LD: Photometric Rate
FDA Status
Approved
Setup Schedule
Monday, Wednesday, Friday
Report Available
3-6 days
Limitations
A hemolyzed specimen is not acceptable as red blood cells contain much more lactate dehydrogenase (LDH) than serum. Causes of hemolysis can include transportation via pneumatic tube, vigorous mixing, or traumatic venipuncture. Tubes should be void of air bubbles to prevent minor hemolysis. LDH activity is one of the most sensitive indicators of in vitro hemolysis. Hemolysis causes anomalous elevation of LDH-I such that any ex vivo hemolysis must be strictly avoided.
Testing should be used with caution in patients with chronic hemolytic anemias such as sickle cell disease as LDH levels may be falsely elevated due to their clinical status.
Freezing or prolonged storage at 4 degrees C (>12 hours) causes LDH-V to be lost.
Elevations of intermediate forms, LDH-II through LDH-IV, are rarely used to define a tissue of origin and such reports are largely anecdotal.
While increases in serum LDH also are seen following a myocardial infarction, the test has been replaced by the determination of troponin (TRPS / Troponin T, 5th Generation, Plasma).
Testing should be used with caution in patients with chronic hemolytic anemias such as sickle cell disease as LDH levels may be falsely elevated due to their clinical status.
Freezing or prolonged storage at 4 degrees C (>12 hours) causes LDH-V to be lost.
Elevations of intermediate forms, LDH-II through LDH-IV, are rarely used to define a tissue of origin and such reports are largely anecdotal.
While increases in serum LDH also are seen following a myocardial infarction, the test has been replaced by the determination of troponin (TRPS / Troponin T, 5th Generation, Plasma).
Reference Range
Included with report
Clinical Significance
Investigating a variety of diseases involving the heart, liver, muscle, kidney, lung, and blood
Differentiating heart-synthesized lactate dehydrogenase (LDH) from liver and other sources
Investigating unexplained causes of LDH elevations
Detection of macro-LDH
Differentiating heart-synthesized lactate dehydrogenase (LDH) from liver and other sources
Investigating unexplained causes of LDH elevations
Detection of macro-LDH
Performing Laboratory
Mayo Clinic Laboratories, Rochester, Minnesota
Additional Information
Lactate Dehydrogenase (LDH) Isoenzymes, Serum
