| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Lyme Disease Antibodies (IgG, IgM) Immunblot
MessageSendout, Mayo test code: LYWB
Test Code
LAB5210316
Alias/See Also
Lyme Disease Antibody, Western Blot Assay, Serum
Western Blot, Lyme Disease
Western Blot Assay
Lyme Western Blot
Lyme Disease Antibody, ImmunoBlot Assay, Serum
Lyme ImmunoBlot
ImmunoBlot Assay, Lyme Disease
Borrelia burgdorferi
LYWB
Western Blot, Lyme Disease
Western Blot Assay
Lyme Western Blot
Lyme Disease Antibody, ImmunoBlot Assay, Serum
Lyme ImmunoBlot
ImmunoBlot Assay, Lyme Disease
Borrelia burgdorferi
LYWB
CPT Codes
86617 x 2
Preferred Specimen
0.75 mL serum from a gold serum gel tube
Minimum Volume
0.5 mL
Other Acceptable Specimens
Red tube
Instructions
Centrifuge and aliquot serum into a plastic vial.
Transport Temperature
Refrigerated
Specimen Stability
Refrigerated (preferred): 14 days
Frozen: 30 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis: Reject
Gross lipemia: Reject
Gross icterus: Reject
Heat-inactivated specimen: Reject
Gross lipemia: Reject
Gross icterus: Reject
Heat-inactivated specimen: Reject
Methodology
Immunoblot
FDA Status
Approved
Setup Schedule
Monday, Wednesday, Friday
Report Available
1-4 days
Limitations
The immunoblot result may be negative in specimens that are weakly positive by enzyme immunoassay or in patients with early Lyme disease.
Test results should be used in conjunction with clinical evaluation and information related to tick exposure.
A negative test result does not necessarily rule out current or recent infection. The specimen may have been collected before demonstrable antibody developed. Patients with early disease often have serum antibody titers below the diagnostic threshold for several weeks following disease onset.
Test results from pregnant women or patients who are immunosuppressed may be difficult to interpret.
Positive test results may not be valid in persons who have received blood or blood product transfusions within the past several months.
Antibiotic therapy administered early following exposure or disease onset may suppress the antibody response to the point that diagnostic threshold levels are never attained.
Lyme disease serology should not be used for monitoring treatment response, as IgG can remain detectable for years post-resolution of infection.
False-positive reactions may occur with patients with other spirochetal diseases (syphilis, yaws, pinta, relapsing fever, or leptospirosis), recent Epstein-Barr virus infection (ie, infectious mononucleosis), influenza, autoimmune disorders (eg, present of extractable nuclear antigens), multiple sclerosis, or amyotrophic lateral sclerosis.
Test results should be used in conjunction with clinical evaluation and information related to tick exposure.
A negative test result does not necessarily rule out current or recent infection. The specimen may have been collected before demonstrable antibody developed. Patients with early disease often have serum antibody titers below the diagnostic threshold for several weeks following disease onset.
Test results from pregnant women or patients who are immunosuppressed may be difficult to interpret.
Positive test results may not be valid in persons who have received blood or blood product transfusions within the past several months.
Antibiotic therapy administered early following exposure or disease onset may suppress the antibody response to the point that diagnostic threshold levels are never attained.
Lyme disease serology should not be used for monitoring treatment response, as IgG can remain detectable for years post-resolution of infection.
False-positive reactions may occur with patients with other spirochetal diseases (syphilis, yaws, pinta, relapsing fever, or leptospirosis), recent Epstein-Barr virus infection (ie, infectious mononucleosis), influenza, autoimmune disorders (eg, present of extractable nuclear antigens), multiple sclerosis, or amyotrophic lateral sclerosis.
Reference Range
Included with report
Clinical Significance
Aiding in the diagnosis of systemic Lyme disease
This test should not be used as a screening assay.
This test should not be used as a screening assay.
Performing Laboratory
Mayo Clinic Laboratories, Rochester, Minnesota
Additional Information
Lyme Disease Antibody, Immunoblot, Serum
