A B C D E F G H I J K L M N O P Q R S T U V W X Y Z #

ADAMTS13 Evaluation

Message
Sendout, Machaon test code: P3336


Test Code
LAB5210235


Alias/See Also
Metalloprotease
Von Willibrand Factor Cleaving Protease
protease activity inhibitor for TTP
microangiopathy, thrombotic microangiopathy
VWF protease activity andinhibitor
P3336


CPT Codes
85397

Preferred Specimen
Blue top 3.2% Sodium Citrate


Patient Preparation
Specimen must be drawn prior to replacement therapy.

Minimum Volume
1 mL plasma


Instructions
Lab: Specimen must be spun within 4 hours of collection
Processing ADAMTS-13:
1. Spin blue top at 7200 rpm for 2 minutes. Use Coag Centrifuges in Specimen Processing.
2. Label aliquot tube (included in kit).
3. Transfer at least 1 ml of plasma into the aliquot tube, seal aliquot tube with lid/parafilm.
4. Hold specimen at room temperature. 


Transport Temperature
Room Temp(Plasma)


Specimen Stability
Room Temp.: 7 days
Refrigerated: 7 days
Frozen (-20C): 2 weeks
Frozen (-80C): 6 months


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Thawed in transit if shipped frozen, refrozen, clotted sample


Methodology
ELISA

FDA Status
Approved

Setup Schedule
24 hour turnaround time 


Limitations
Samples containing EDTA cannot be used because EDTA is a strong inhibitor of ADAMTS13 function. Hemolysis does not affect assay performance with samples containing up to 200mg/dL of hemoglobin. Lipemia does not affect assay performance with samples containing up to 300mg/dL of lipids. Icterus does not affect assay performance with samples containing up to 15mg/dL of bilirubin (both conjugated and unconjugated).


Reference Range
Included with report


Performing Laboratory
Machaon Diagnostic, Inc. 

Additional Information
ADAMTS13 Activity


The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.