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Heparin Anti-XA, Unfractionated
MessageCoagulation
Test Code
LAB5210289
Alias/See Also
Anti-XA (UF)
Anti-XA(UFH)
Unfractionated Heparin
Anti-XA(UFH)
Unfractionated Heparin
CPT Codes
85520
Preferred Specimen
Blue top tube 3.2% citrated tube, plasma
Minimum Volume
2.7 mL whole blood filled to the designated line on specimen container (blue top tube, citrate)
Other Acceptable Specimens
1.8mL Low Volume blue top tube, citrated
Instructions
Do not place Blue top tube on ice.
LAB: Centrifuge in validated Platelet Poor Plasma Centrifuges only
LAB: Centrifuge in validated Platelet Poor Plasma Centrifuges only
Transport Container
Processed Platelet Poor Plasma, preferably frozen
Transport Temperature
Processed Platelet Poor Plasma:
Room Temp – 4 hours
Refrigerated – 4 hours
Frozen – 1 month
Room Temp – 4 hours
Refrigerated – 4 hours
Frozen – 1 month
Specimen Stability
Whole Blood: Ambient 1 hour
Plasma separated from whole blood 2-8°C 4 hours
Plasma separated from whole blood -20°C 1 month
Plasma separated from whole blood -70°C 6 months
Plasma may be frozen and Maintained at -70oC for two freeze/thaw cycle. Note: Do not place Blue top tube on ice
Plasma separated from whole blood 2-8°C 4 hours
Plasma separated from whole blood -20°C 1 month
Plasma separated from whole blood -70°C 6 months
Plasma may be frozen and Maintained at -70oC for two freeze/thaw cycle. Note: Do not place Blue top tube on ice
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Underfilled tube - Quantity not Sufficient (QNS)
Clotted specimen
Clotted specimen
Methodology
Heparin is analyzed as a complex with antithrombin present in the sample. The concentration of this complex is dependent on the availability of the patient's endogenous antithrombin. When the heparin - antithrombin complex is formed, two competing reactions take place: Factor Xa is neutralized by the heparin-antithrombin complex Residual Factor Xa is quantified with a synthetic chromogenic substrate. The paranitroaniline released is monitored kinetically at 405 nm and is inversely proportional to the heparin level in the sample. In order to reduce the influence from heparin antagonists, such as platelet factor 4 (PF4), dextran sulfate is included in the reaction mixture
FDA Status
Cleared
Setup Schedule
Daily
Report Available
STAT: 1 hour
Routine: Same Day
Routine: Same Day
Limitations
Hemoglobin up to 300 mg/dL
Bilirubin up to 20 mg/dL
Triglyceride up to 800 mg/dL.
Bilirubin up to 20 mg/dL
Triglyceride up to 800 mg/dL.
Reference Range
0.3 - 0.7 U/mL
Clinical Significance
To measure biological activity of Heparin as antithrombiotic drug
Performing Laboratory
HHN/HHI