Syphilis Antibody Cascading Reflex

Message

Sendout, Mayo test code: SYPH1

Test Code

LAB5210949

Alias/See Also

ART (Automated Reagin Test)
Automated Reagin Test (ART)
Rapid Plasma Reagin (RPR)
RPR (Rapid Plasma Reagin)
Syphilis G, Serum
Syphilis Serology (RPR)
T. pallidum (Treponema pallidum)
Treponema pallidum (T. pallidum)
SYPH1

CPT Codes
86780, 86592 (if appropriate), 86593 (if appropriate), 86780 (if appropriate)

Includes

If the syphilis IgG result is reactive or equivocal, then the rapid plasma reagin (RPR) screen will be performed at an additional charge.

If the RPR screen is positive, then the RPR titer will be performed at an additional charge.

If the RPR screen is negative, then syphilis antibody Treponema pallidum particle agglutination testing will be performed at an additional charge.

Preferred Specimen

1.5 mL serum from a gold serum gel tube

Minimum Volume

1 mL

Other Acceptable Specimens

Red tube

Instructions

Centrifuge and aliquot serum into a plastic vial.

Transport Container

Plastic vial

Transport Temperature

Frozen

Specimen Stability

Frozen: 14 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis: Reject
Gross lipemia: Reject
Heat-inactivated specimen: Reject

Methodology
SYPH1: Enzyme Immunoassay (EIA) RRPRS, RRPRQ: Flocculation/Agglutination RTPPA: Particle Agglutination

FDA Status
Approved

Setup Schedule

Monday through Friday, Sunday

Report Available

1-4 days

Limitations

Despite active syphilis, serologic tests may be negative in severely immunosuppressed patients such as those with AIDS.

In very early cases of primary syphilis, serology tests for syphilis may be negative.

In cases of untreated, late or latent syphilis, the result of rapid plasma reagin may be negative. However, the syphilis screening by enzyme immunoassay and Treponema pallidum particle agglutination should be positive. A thorough clinical and historical evaluation should be performed to determine if treatment for latent syphilis is required.

Results should be considered in the context of all available clinical and laboratory data.

Reference Range

Included with report

Clinical Significance

Aid for the diagnosis of infection with Treponema pallidum using an algorithmic approach

Routine prenatal screening

This test is not offered as a screening or confirmatory test for blood donor specimens.

This test is not useful for diagnosis of congenital syphilis.

Performing Laboratory
Mayo Clinic Laboratories, Rochester, Minnesota

Additional Information
Syphilis IgG with Reflex, Enzyme Immunoassay, Serum

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.