Proteinase 3 Antibody

Message

Sendout, Mayo test code: PR3

Test Code

LAB5210332

Alias/See Also

Autoantibodies to Proteinase 3
c-ANCA (Antineutrophil Cytoplasmic Antibodies)
cANCA (Antineutrophil Cytoplasmic Antibodies)
Cytoplasmic Neutrophil Antibodies
Neutrophil Cytoplasmic Antibodies
pANCA (Perinuclear Antineutrophil Cytoplasmic Antibodies)
Perin

CPT Codes
83516

Preferred Specimen

0.5 mL serum from a red tube

Minimum Volume

0.35 mL

Other Acceptable Specimens

Serum gel tube

Instructions

Centrifuge and aliquot serum into a plastic vial.

Transport Container

Plastic vial

Transport Temperature

Refrigerated

Specimen Stability

Refrigerated (preferred): 21 days
Frozen: 21 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis: Reject
Gross lipemia: Reject
Gross icterus: OK

Methodology
Multiplex Flow Immunoassay

FDA Status
Approved

Setup Schedule

Monday through Saturday

Report Available

1-3 days

Limitations

While the presence of proteinase 3 antineutrophil cytoplasmic antibodies (PR3 ANCA) is highly specific for Wegener granulomatosis (WG), it is recommended that positive test results obtained by immunoassay be confirmed by another testing method.(4) This is best accomplished by testing for cytoplasmic ANCA (cANCA) and perinuclear ANCA (pANCA) by indirect immunofluorescence microscopy (ANCA / Cytoplasmic Neutrophil Antibodies, Serum). Alternately, VASC / Antineutrophil Cytoplasmic Antibodies Vasculitis Panel, Serum includes tests for PR3 ANCA, myeloperoxidase antibodies, and, if indicated, cANCA and pANCA. This panel is recommended for the initial diagnostic evaluation of patients clinically suspected of having systemic vasculitis. The simultaneous presence of PR3 ANCA and cANCA has a specificity greater than 99% for WG.(3)

Reference Range

Included with report

Clinical Significance

Evaluating patients suspected of having Wegener granulomatosis (WG)

Distinguishing between WG and other forms of vasculitis, in conjunction with:
-MPO / Myeloperoxidase Antibodies, IgG, Serum
-ANCA / Cytoplasmic Neutrophil Antibodies, Serum (may be obtained as VASC / Antineutrophil Cytoplasmic Antibodies Vasculitis Panel, Serum)

May be useful to follow treatment response or to monitor disease activity in patients with myeloperoxidase antibodies

Performing Laboratory
Mayo Clinic Laboratories, Rochester, Minnesota

Additional Information
Proteinase 3 Antibodies, IgG, Serum

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.