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RETIC COUNT
MessageHematology
Test Code
LAB296
Alias/See Also
Reticulocyte
Retic-Hemoglobin
Retic-Hemoglobin
CPT Codes
85046
Includes
Retic Abs
Retic %
Immature Retic Fraction (IRF%)
Reticulocyte Hemoglobin Content (Ret-He)
Retic %
Immature Retic Fraction (IRF%)
Reticulocyte Hemoglobin Content (Ret-He)
Preferred Specimen
2.5 mL whole blood (lavender top tube, EDTA)
May combine collection with CBC order
May combine collection with CBC order
Minimum Volume
0.6 mL
Other Acceptable Specimens
Microtainer fingerstick/heelstick (neonates)
Instructions
**Testing must be completed within 24 hours of collection.
Transport Temperature
Never place whole blood on ice or transport frozen.
Specimen Stability
Room Temp 24 hours
Refrigerated 48 hours
Allow refrigerated samples to come to room temperature and mix well before analysis.
Refrigerated 48 hours
Allow refrigerated samples to come to room temperature and mix well before analysis.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolyzed
Contaminated
Clotted
QNS
Contaminated
Clotted
QNS
Methodology
The analyzer performs hematology analysis according to the hydrodynamic focusing (DC Detection), flow cytometry method (semiconductor laser), and SLS-hemoglobin method. The reticulocytes (RET) are all evaluated using flow cytometry with a semiconductor laser exploiting the differences in cell size, complexity, and RNA / DNA content
Report Available
Same day
Limitations
Severely hemolyzed samples (in vitro) falsely decrease RBC and hematocrit. Recollect hemolyzed specimens.
Severely icteric samples may falsely elevate the HGB value and related indices.
Extremely elevated WBC counts may cause turbidity and falsely increase the hemoglobin, RBC and HCT values.
Severely icteric samples may falsely elevate the HGB value and related indices.
Extremely elevated WBC counts may cause turbidity and falsely increase the hemoglobin, RBC and HCT values.
Reference Range
Retic: 0.9-1.9%
IRF: 2.30-13.40
RET-HE: 29-37pg
IRF: 2.30-13.40
RET-HE: 29-37pg
Clinical Significance
Ret-He is a sensitive early indicator of iron deficiency that assesses the amount of iron available to make hemoglobin and support erythropoiesis. Low Ret-He indicates actual/functional iron deficiency and provides information as reliable as iron profiles.
Performing Laboratory
HHN / HHI Laboratory