Glucose-6-Phosphate Dehydrogenase,Qual

Message

Hematology

Test Code

LAB10035

Alias/See Also

Glucose-6-Phosphate Dehydrogenase Qualitative
G6PD

CPT Codes
82960

Preferred Specimen

2.5 mL whole blood (lavender top tube, EDTA) For infants 200 µL (microliter) EDTA microtainer

Minimum Volume

2.5 mL

Other Acceptable Specimens

Whole blood collected with Heparin or ACD

Instructions

LAB: Store whole blood in refrigerator in hematology Dept weekends and off-hours.

Specimen Stability

Refrigerated 2 - 8° C: 1 week

Methodology
Qualitative, visual fluorescence, screening procedure for the detection of G-6-PD deficiency in whole blood. In the procedure, the reaction mixture containing glucose-6-phosphate, NADP and a small amount of blood is incubated for a few minutes. Drops from the mixture are spotted onto filter paper at zero time and at 5-minute intervals. The spots are then visually inspected under long wave UV light. Development of fluorescence will be observed with normal blood. No appreciable fluorescence appears when G-6-PD is deficient.

Setup Schedule

Test performed 0600 - 1100 Monday through Friday only.

Report Available

Test performed 0600 - 1100 Monday through Friday only. Report available upon completion.

Limitations

Leukocytes and platelets may be rich in G-6-PDH and may cause some interference in the assay if present beyond normal levels. Therefore, higher than normal levels of G-6-PD could occur in cases of leukocytosis and thrombocytosis.

Young red blood cells (reticulocytes) have higher G-6-PD levels than mature erythrocytes. A false-negative result, indicating the sample is normal, may be obtained in individuals with an anemia and resulting reticulocytosis.

Reference Range

Normal

Clinical Significance

Glucose-6-phosphate deficiency is an X-linked disorder of carbohydrate metabolism, which can result in either congenital nonspherocytic hemolytic anemia or a drug- induced hemolytic anemia. Substances capable of inducing hemolysis in affected individuals include fava beans, anti-malarial drugs, aspirin, vitamin K analogues, sulfonamides, and other compounds. This anomaly is found predominately in Mediterranean peoples, Southeast Asians, Africans, and African Americans.

Performing Laboratory
HHN

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.