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PROTEIN, URINE, 24HR
MessageChemistry
Test Code
LAB441
Alias/See Also
24hr Total Protein, Total Protein 24hr, 24 hour
CPT Codes
84156
Includes
Total Urine Protein OutPut for 24 hr period
Preferred Specimen
24-hour urine collection
Minimum Volume
300 uL urine
Instructions
Refrigerate urine during collection. Submit entire collection to Laboratory with completed Order requisition
LAB: Properly labeled Timed collection received with LIS Order Requisition.
Measure urine volume and record on Order Requisition.
Mix well, aliquot 5 mL and centrifuge.
Take spun urine aliquot and Order Requisition to Tech for testing.
Tech will verify Total Volume & HOURS of collection on Order Requisition:
Date Urine Collection Started
Date Urine Collection Ends
Time Urine Collection Started
Time Urine Collection Ends
LAB: Properly labeled Timed collection received with LIS Order Requisition.
Measure urine volume and record on Order Requisition.
Mix well, aliquot 5 mL and centrifuge.
Take spun urine aliquot and Order Requisition to Tech for testing.
Tech will verify Total Volume & HOURS of collection on Order Requisition:
Date Urine Collection Started
Date Urine Collection Ends
Time Urine Collection Started
Time Urine Collection Ends
Specimen Stability
Room Temperature 18–28 °C (64–82 °F) ≤ 4 hours
Refrigerated 2–8 °C (36–46 °F) ≤ 3 days
Refrigerated 2–8 °C (36–46 °F) ≤ 3 days
Methodology
Colorimetric
FDA Status
Approved
Setup Schedule
Everyday
Report Available
Same day
Limitations
Low levels (<35 mg/dL) of Bence Jones or light chain proteins may not be detected. Elevated levels of Bence Jones or
light chain proteins may predict approximately 40% lower concentrations than pyrogallol red or turbidimetric methods.
Refer to the Recovery table.
• Very dilute urine samples (specific gravity ≤1.010) may yield artificially high 24-hour protein concentrations in large
volume (>2500 mL) samples.
• Very concentrated (specific gravity ≥1.025) urine samples may yield artificially low 24-hour protein concentrations in
large volume (>2500 mL) samples.
• Certain drugs and clinical conditions are known to alter urine protein concentration in vivo. For additional information,
refer to one of the published summaries.
light chain proteins may predict approximately 40% lower concentrations than pyrogallol red or turbidimetric methods.
Refer to the Recovery table.
• Very dilute urine samples (specific gravity ≤1.010) may yield artificially high 24-hour protein concentrations in large
volume (>2500 mL) samples.
• Very concentrated (specific gravity ≥1.025) urine samples may yield artificially low 24-hour protein concentrations in
large volume (>2500 mL) samples.
• Certain drugs and clinical conditions are known to alter urine protein concentration in vivo. For additional information,
refer to one of the published summaries.
Reference Range
42-225 MG/DAY
Performing Laboratory
Hoag Newport/ Hoag Irvine
