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Hepatitis B Surface Antigen
MessageChemistry
Test Code
LAB471
Alias/See Also
HBsAg, Hepatitis B Surface AG
CPT Codes
87340
Preferred Specimen
Gold top (serum separator tube)
Minimum Volume
Whole blood: 800 uL (TWO full red-top microtainers).
Serum/plasma: 400 uL
Serum/plasma: 400 uL
Other Acceptable Specimens
Red Top tube acceptable
Instructions
Testing also included in HEPATITIS PANEL, ACUTE
All specimens Reactive for Hepatitis B Surface Antigen performed in-house will be sent to MYO for confirmation: "Hepatitis B Surface Ag Reflex (MYO: HBAG, order as Miscellaneous)". If indicated, Mayo Lab may additionally reflex "Hep B Surface Antigen Conf" (MYO: HBGNT, order as Miscellaneous when performed). Reflex testing is at an additional charge.
Allow two additional days turn around time. If reflex testing is inidcated, CLS to take sample to Reference Desk for processing HBAG to MYO.
All specimens Reactive for Hepatitis B Surface Antigen performed in-house will be sent to MYO for confirmation: "Hepatitis B Surface Ag Reflex (MYO: HBAG, order as Miscellaneous)". If indicated, Mayo Lab may additionally reflex "Hep B Surface Antigen Conf" (MYO: HBGNT, order as Miscellaneous when performed). Reflex testing is at an additional charge.
Allow two additional days turn around time. If reflex testing is inidcated, CLS to take sample to Reference Desk for processing HBAG to MYO.
Transport Temperature
Refrigerated
Specimen Stability
Ambient: 8 hours
Refrigerated: 48 hours
Frozen: 14 days
Refrigerated: 48 hours
Frozen: 14 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Do not use turbid specimens
Methodology
Immunometric
FDA Status
Approved
Setup Schedule
Everyday
Report Available
Same day
Limitations
Individuals recently vaccinated for hepatitis B may give a transient positive result for HBsAg because of its
presence in the vaccine.
HBsAg results should only be used and interpreted in the context of the overall clinical picture. A negative test
result does not exclude the possibility of exposure to or infection with hepatitis B virus. Levels of HBsAg may
be undetectable both in early infection and late after infection. In rare cases HBsAg tests do not detect certain
HBV mutant strains
presence in the vaccine.
HBsAg results should only be used and interpreted in the context of the overall clinical picture. A negative test
result does not exclude the possibility of exposure to or infection with hepatitis B virus. Levels of HBsAg may
be undetectable both in early infection and late after infection. In rare cases HBsAg tests do not detect certain
HBV mutant strains
Reference Range
Negative
Performing Laboratory
Hoag Newport/ Hoag Irvine
Additional Information
Link to Reflex test: Hepatitis B Surface Ag Reflex
