FIBRINOGEN

Message

Coagulation

Test Code

LAB314

Alias/See Also

FIBR

CPT Codes
85384

Preferred Specimen

Blue top 3.2% citrated tube, plasma
Note: Do NOT place blue top tube on ice

Minimum Volume

2.7 mL whole blood filled to the designated line on specimen container (blue top tube, citrate)

Other Acceptable Specimens

1.8mL Low Volume blue top tube, citrated (microtainer)

Instructions

LAB: Centrifuge in validated Platelet Poor Plasma Centrifuges for 2 minutes at 7200 rpm. Specimen to be delivered to Coagulation department STAT. Notify CLS upon arrival.
**Testing must be completed within 4 hours of collection.

Note: If unable to deliver whole blood to testing laboratory within stability time, platelet poor plasma should be aliquoted and placed in freezer. Centrifuge light blue-top for 2 minutes at 7200 rpm (STAT Coag centrifuge) within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge aliquoted plasma a second time and transfer platelet-poor plasma into a new plastic vial and freeze at -20℃ if delay longer than 1 hour. Once thawed, specimen must be tested within 2 hours.

Freeze aliquot at -20℃ for at least 1 hour when sending to alternate campus for testing.

Transport Container

Ambient: blue top tube, whole blood (4 hours)
Frozen: processed platelet poor plasma (Preferred)

Transport Temperature

Ambient: whole blood (stable 4 hours)
Frozen (-20℃): plasma frozen for at least 1 – 2 hours

Specimen Stability

Whole Blood: Ambient 4 hours
Plasma separated from whole blood 2-8°C 4 hours
Plasma separated from whole blood -20°C 2 weeks
Plasma separated from whole blood -70°C 6 months

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolyzed, clotted specimen, contaminated, not enough volume, overfilled tube, whole blood specimen transported on ice

Methodology
Fibrinogen is a plasma protein that is converted from a soluble protein to an insoluble polymer by the action of thrombin, resulting in the formation of a fibrin clot. Fibrinogen in the diluted test sample is converted to fibrin by the addition of an excess amount of thrombin. The resulting clotting time value is measured. The log of the clotting time value is inversely proportional to the log of the fibrinogen concentration. A fibrinogen reference curve is plotted from the clotting time results of the known referenced plasma dilutions having different fibrinogen values. The concentration of fibrinogen in patient plasma samples is determined by comparing clotting time values to the reference curve.

Report Available

Same Day

Limitations

Hemoglobin up to 375 mg/dL
Bilirubin up to 23 mg/dL
Triglycerides up to 880 mg/dL
Heparin up to 2 U/mL

Reference Range

200-393 mg/dL
Critical Value: <100 mg/dL

Clinical Significance

Fibrinogen is made in your liver and helps your blood clot. Low fibrinogen may make it difficult for your blood to clot.

Performing Laboratory
HHN/HHI

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.