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Factor VIII, Activity
MessageCoagulation
Test Code
LAB306
Alias/See Also
FVIII
F8
Factor 8
F8
Factor 8
CPT Codes
85240
Preferred Specimen
2.7 mL whole blood (blue top tube, citrate) **TUBE MUST BE FULL**
Minimum Volume
2.7 mL
Instructions
Specimen to be delivered to Coagulation department STAT. Notify CLS upon arrival. **Testing must be completed within 4 hours of collection.
LAB: If testing is not to be done within 2 hours, centrifuge light blue-top for 2 minutes at 7200 rpm (STAT coag. centrifuge) within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial.
Centrifuge aliquoted plasma a second time and transfer platelet-poor plasma into a new plastic vial and place in 2-8℃ refrigerator or freeze -20℃ if delay longer than 1 hour.
Freeze aliquot at -20oC for at least 1 hour when sending to alternate campus for testing.
LAB: If testing is not to be done within 2 hours, centrifuge light blue-top for 2 minutes at 7200 rpm (STAT coag. centrifuge) within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial.
Centrifuge aliquoted plasma a second time and transfer platelet-poor plasma into a new plastic vial and place in 2-8℃ refrigerator or freeze -20℃ if delay longer than 1 hour.
Freeze aliquot at -20oC for at least 1 hour when sending to alternate campus for testing.
Specimen Stability
Whole blood sample stability
Ambient: 2 hrs
Centrifuged and separated plasma (see instructions)
Refrigerated 2-8oC: 4 hours
Frozen: -20oC: 2 weeks
Frozen: -70oC: 6 months
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolyzed, contanminated, clotted, not enough volume
Report Available
Same Day or within 24 hours
Reference Range
50 - 150%
Performing Laboratory
HHN
