| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Factor VIII, Activity
MessageCoagulation
Test Code
LAB306
Alias/See Also
FVIII F8 Factor 8
Factor 8 Assay
Factor 8 Assay
CPT Codes
85240
Preferred Specimen
Blue top 3.2% citrated tube, plasma
Note: Do NOT place blue top tube on ice
Note: Do NOT place blue top tube on ice
Minimum Volume
| 2.7 mL whole blood filled to the designated line on specimen container (blue top tube, citrate) |
Other Acceptable Specimens
1.8 mL Low Volume blue top tube, citrated (microtainer)
Instructions
LAB: Centrifuge in validated Platelet Poor Plasma Centrifuge for 2 minutes at 7200 rpm. Specimen to be delivered to Coagulation department STAT. Notify CLS upon arrival.
**Testing must be completed within 4 hours of collection.
Note: If unable to deliver whole blood to testing laboratory within stability time, platelet poor plasma should be aliquoted and placed in freezer. Centrifuge light blue-top for 2 minutes at 7200 rpm (STAT Coag centrifuge) within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge aliquoted plasma a second time and transfer platelet-poor plasma into a new plastic vial and freeze at -20℃ if delay longer than 1 hour. Once thawed, specimen must be tested within 2 hours.
Freeze aliquot at -20℃ for at least 1 hour when sending to alternate campus for testing.
**Testing must be completed within 4 hours of collection.
Note: If unable to deliver whole blood to testing laboratory within stability time, platelet poor plasma should be aliquoted and placed in freezer. Centrifuge light blue-top for 2 minutes at 7200 rpm (STAT Coag centrifuge) within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge aliquoted plasma a second time and transfer platelet-poor plasma into a new plastic vial and freeze at -20℃ if delay longer than 1 hour. Once thawed, specimen must be tested within 2 hours.
Freeze aliquot at -20℃ for at least 1 hour when sending to alternate campus for testing.
Transport Temperature
Ambient: whole blood (stable 2 hours)
Frozen (-20℃): plasma frozen for at least 1 – 2 hours
Frozen (-20℃): plasma frozen for at least 1 – 2 hours
Specimen Stability
Whole blood sample stability
Ambient: 2 hrs
Centrifuged and separated plasma (see instructions)
Refrigerated 2-8oC: 4 hours
Frozen: -20oC: 2 weeks
Frozen: -70oC: 6 months
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolyzed, clotted specimen, contaminated, not enough volume, overfilled tube, whole blood specimen transported on ice
Report Available
Same Day or within 24 hours
Reference Range
50 - 150%
Performing Laboratory
HHN
