A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
CBC NO DIFFERENTIAL
MessageHematology
Test Code
LAB294
Alias/See Also
Complete Blood Count
CBC
Hemogram
CBC
Hemogram
CPT Codes
85027
Includes
WBC, RBC, HGB, HCT, MCV, MCH, MCHC, RDW-CV, RDW-SD, PLT, MPV, NRBC%
May reflex a Reticulocyte Battery (Retic ABS, Retic %, IRF%, Ret-He) or Immature Platelet Fraction
May reflex a Reticulocyte Battery (Retic ABS, Retic %, IRF%, Ret-He) or Immature Platelet Fraction
Preferred Specimen
2.5 mL whole blood (lavender top tube, EDTA)
Minimum Volume
1.0mL
Other Acceptable Specimens
Microtainer fingerstick/heelstick (neonates)
Instructions
**Testing must be completed within 24 hours of collection.
Transport Temperature
Never place whole blood on ice or transport frozen.
Specimen Stability
Room Temp 24 hours
Refrigerated 48 hours
Allow refrigerated samples to come to room temperature and mix well before analysis.
Refrigerated 48 hours
Allow refrigerated samples to come to room temperature and mix well before analysis.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolyzed
Contaminated
Clotted
QNS
Contaminated
Clotted
QNS
Methodology
The analyzer performs hematology analysis according to the hydrodynamic focusing (DC Detection), flow cytometry method (semiconductor laser), and SLS-hemoglobin method. The device counts and sizes red blood cells (RBC) and platelets (PLT) using electronic resistance detection. Hematocrit (HCT) is measured as a ratio of the total RBC volume to whole blood using cumulative pulse height detection. Hemoglobin (HGB) is converted to SLS-hemoglobin and read photometrically. The white blood cell (WBC) count, differential (DIFF), reticulocytes (RET), nucleated red blood cells (NRBC) and fluorescent platelets (PLT-F) are all evaluated using flow cytometry with a semiconductor laser exploiting the differences in cell size, complexity, and RNA / DNA content. Forward scattered light provides information on blood cell size and lateral scattered light provides information on the cell interior such as the size of the nucleus. Lateral fluorescent light intensity increases as the concentration of the stain becomes higher. By measuring the intensity of the fluorescence emitted, information is obtained on the degree of blood cell staining. Fluorescent light is emitted in all directions. The Sysmex XN detects the fluorescent light that is emitted sideways.
Setup Schedule
Same day. Available STAT.
Report Available
Same day
Limitations
Severely hemolyzed samples (in vitro) falsely decrease RBC and hematocrit. Recollect hemolyzed specimens.
Severely icteric samples may falsely elevate the HGB value and related indices.
Extremely elevated WBC counts may cause turbidity and falsely increase the hemoglobin, RBC and HCT values.
Platelet clumping and/or "platelet satellitism" may occur in specimens collected in EDTA. Methods for handling samples with platelet clumping or "platelet satellitism" include: vortexing the original sample and reanalyzing or redrawing the patient sample in a blue top and testing within 30 min of draw.
Severely icteric samples may falsely elevate the HGB value and related indices.
Extremely elevated WBC counts may cause turbidity and falsely increase the hemoglobin, RBC and HCT values.
Platelet clumping and/or "platelet satellitism" may occur in specimens collected in EDTA. Methods for handling samples with platelet clumping or "platelet satellitism" include: vortexing the original sample and reanalyzing or redrawing the patient sample in a blue top and testing within 30 min of draw.
Reference Range
Refer to CBC report for individual Reference Ranges
Clinical Significance
The Sysmex XN Hematology module is a quantitative automated hematology analyzer for in vitro diagnostic use in determining 31 whole blood diagnostic parameters and seven body fluid diagnostic parameters. Examination of the numerical and/or morphological findings of the complete blood count by the physician are useful in the diagnosis of disease states such as anemias, leukemias, allergic reactions, viral, bacterial, and parasitic infections.
Performing Laboratory
HHN/HHI