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HIV-1/2 Qualitative RNA
MessageSendout, Mayo test code: HIP12
Test Code
LAB5211783
Alias/See Also
Acquired Immune Deficiency Syndrome (AIDS)
AIDS (Acquired Immune Deficiency Syndrome)
HIV (Human Immunodeficiency Virus)
HIV-1 Detection
HIV-1 PCR
HIV-1 RNA
HIV-2 Detection
HIV-2 PCR
HIV-2 RNA
HIP12
AIDS (Acquired Immune Deficiency Syndrome)
HIV (Human Immunodeficiency Virus)
HIV-1 Detection
HIV-1 PCR
HIV-1 RNA
HIV-2 Detection
HIV-2 PCR
HIV-2 RNA
HIP12
CPT Codes
87535, 87538
Preferred Specimen
1.5 mL plasma from a lavender EDTA tube.
Minimum Volume
0.8 mL plasma
Instructions
1. Centrifuge blood collection tube and aliquot plasma into plastic vial per collection tube manufacturer's instructions (eg, centrifuge and aliquot within 2 hours of collection for BD Vacutainer tubes).
2. Freeze aliquoted plasma for shipment.
2. Freeze aliquoted plasma for shipment.
Transport Container
Aliquot tube
Transport Temperature
Frozen
Specimen Stability
Plasma EDTA Frozen (preferred): 40 days
Refrigerated: 6 days
Refrigerated: 6 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK
Gross lipemia Reject
Gross icterus OK
Methodology
Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
Setup Schedule
Monday through Saturday
Report Available
1 to 3 days
Limitations
This test is not licensed by the US Food and Drug Administration as a screening test for HIV-1 infection in donors of blood, human cells, tissues, or tissue products.
A single "Undetected" result may not rule out the presence of HIV infection, if the plasma specimen is collected and tested too early after exposure (ie, less than 10 days from exposure) or if patient is infected with a rare HIV variant. Improper storage or processing (ie, incorrect temperature) of the plasma specimen for transport to the testing laboratory may lead to falsely negative viral RNA result.
A single "Undetected" result may not rule out the presence of HIV infection, if the plasma specimen is collected and tested too early after exposure (ie, less than 10 days from exposure) or if patient is infected with a rare HIV variant. Improper storage or processing (ie, incorrect temperature) of the plasma specimen for transport to the testing laboratory may lead to falsely negative viral RNA result.
Reference Range
Included with report
Clinical Significance
Diagnosis of HIV-1 and/or HIV-2 infection in individuals with indeterminate or inconclusive HIV serologic test results
Diagnosis of HIV-1 and/or HIV-2 infection in individuals with acute or early HIV-1 and/or HIV-2 infection
Diagnosis of HIV-1 and/or HIV-2 infection in infants under 18 months of age who are born to HIV-infected mothers
Diagnosis of HIV-1 and/or HIV-2 infection in individuals with acute or early HIV-1 and/or HIV-2 infection
Diagnosis of HIV-1 and/or HIV-2 infection in infants under 18 months of age who are born to HIV-infected mothers
Performing Laboratory
Mayo Clinic Laboratories, Rochester, Minnesota
Additional Information
HIV-1/HIV-2 RNA Detection, Plasma
