Interleukin-2 Receptor Alpha Chain (IL-2Ra/CD25), Soluble

Sendouts, Mayo test code: SIL2R

LAB1238128

CD25
cytokines
IL 2 Receptor
IL-2R
IL2R
Interleukin 2 Receptor
IL2RS
Soluble IL-2 Receptor alpha
Soluble CD25/IL-2 R?
Soluble Interleukin-2 Receptor Alpha
Cytokine
sCD25
SIL2R

0.5 mL plasma from a lavender EDTA tube

0.3 mL

1. Immediately after specimen collection, place tube on wet ice.
2. Centrifuge at 4 degrees C, 1500 x g for 10 minutes.
3. Aliquot plasma a into plastic vial.
4. Freeze specimen within 2 hours of collection.

Plastic vial

Frozen

Frozen: 21 days

Gross hemolysis: Reject
Gross lipemia: Reject
Gross icterus: Reject
Heat-treated specimen: Reject

Thursday

2 to 8 days

Soluble interleukin-2 receptor alpha (sIL-2r alpha) testing should only be used in conjunction with clinical presentation and other laboratory testing as part of a patient's overall assessment. Elevations in sIL-2r alpha can be seen in several inflammatory, malignancy, or infectious conditions involving activation of T-cells, B-cells, granulocytes or natural killer cells. Non-elevated concentration of sIL-2r alpha does not exclude the possibility of infection or other inflammatory condition.

There is substantial overlap in elevated sIL-2r alpha in patients with other chronic inflammatory conditions or infections Soluble interleukin-2 receptor alpha is marker for lymphocyte activation and proliferation; a comprehensive cytokine evaluation, such as CYPAN / Cytokine Panel,

Plasma may be more useful in overall disease assessment or pathophysiology.

Included with report

Measuring the concentration of soluble interleukin-2 receptor alpha (sIL-2r alpha) in plasma

Aids in the diagnosis and evaluation of patients for lymphoproliferative disorders, including autoimmune lymphoproliferative syndrome, hemophagocytic lymphohistiocytosis, and macrophage activation syndrome

May help monitor patients with sarcoidosis or hematologic malignancies with elevated sIL-2r alpha

The interleukin-2 (IL-2) receptor (CD25) is a membrane protein that is upregulated on activated T cells. Its soluble form, IL-2 receptor alpha soluble (sIL-2r alpha, soluble CD25) is notably elevated in lymphoproliferative disorders, such as hemophagocytic lymphohistiocytosis (HLH), autoimmune lymphoproliferative syndrome (ALPS), T-cell related leukemia-lymphoma and other conditions associated with T-cell activation.(1) Based on the HLH-2004 trial, the diagnosis of HLH syndrome should be based on compatible clinical presentation in the context of significantly elevated inflammatory markers, including ferritin, sIL-2r alpha, and CXCL9 (C-X-C motif chemokine ligand 9). sIL-2r alpha elevation at a significant level (2SD above age-adjusted laboratory-specific reference range) is a crucial diagnostic criterion.(2)
 
In addition to activated T cells, IL-2 receptor is also expressed on a certain subset of B cells, Regulatory Teg cells, granulocytes and natural killer (NK) cells.(3) Elevation of sIL-2r alpha can be observed in other chronic inflammatory conditions and malignancies.(4,5) In sarcoidosis patients with cardiac involvement, higher levels of sIL-2r alpha are associated with worse long-term clinical outcomes and increase systemic inflammatory activities.(6) In patients with hematological malignancies that express IL-2 receptors, sIL-2r alpha elevations are associated with poor prognosis or disease relapse.(4,7)