Hepatitis B Surface Antigen Screen, Serum

Message

Sendout, Mayo test code: HBGSN

Test Code

LAB1315

Alias/See Also

HBAG screen
HBs Ag (Hepatitis Bs Antigen) screen
HBV s antigen screen
HBV surface antigen screen
Hepatitis B surface antigen screen
Hepatitis Bs Ag screen
Hepatitis Bs antigen screen
HBGSN

CPT Codes
87340, G0499 (if appropriate), 87341 (if appropriate)

Includes

If hepatitis B surface antigen (HBsAg) screen is reactive with signal-to-cutoff ratio in the range of 1.00 to 100.0 then HBsAg confirmation will be performed at an additional charge.

Preferred Specimen

2 mL serum from a gold serum gel tube

Minimum Volume

0.6 mL

Other Acceptable Specimens

Red tube

Instructions

1. Centrifuge blood collection tube per collection tube manufacturer's instructions (eg, centrifuge and aliquot within 2 hours of collection for BD Vacutainer tubes).
2. Aliquot serum into plastic vial.

Transport Container

Plastic vial

Transport Temperature

Frozen

Specimen Stability

Frozen (preferred): 28 days
Refrigerated: 7 days
Ambient: 24 hours

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis: Reject
Gross lipemia: Reject
Gross icterus: Reject

Methodology
Chemiluminescence Immunoassay

FDA Status
Approved

Setup Schedule

Monday through Saturday

Report Available

1-3 days

Limitations

Positive screen results (ie, signal-to-cutoff ratio >100.0) without need for confirmation testing should be interpreted in conjunction with test results of other hepatitis B virus serologic markers (eg, hepatitis B surface antibody [anti-HBs], hepatitis B core antibody [anti-HBc] total, and anti-HBc IgM).

Positive hepatitis B surface antigen (HBsAg) test results should be reported by the healthcare provider to the State Department of Health, as required by law in some states.

Individuals, especially neonates and children, who recently received hepatitis B vaccination may have transient positive HBsAg test results because of the large dose of HBsAg used in the vaccine relative to the individual's body mass.

Performance characteristics have not been established for the following specimen characteristics:
-Grossly icteric (total bilirubin level of >20 mg/dL)
-Grossly lipemic (triolein level of >3000 mg/dL)
-Grossly hemolyzed (hemoglobin level of >500 mg/dL)
-Containing particulate matter
-Cadaveric specimens

Reference Range

Included with report

Clinical Significance

Diagnosis of acute, recent, or chronic hepatitis B viral (HBV) infection

Determination of chronic HBV infection status

This test is not offered as a screening or confirmatory test for blood donor specimens.

This test is not useful for diagnosis of hepatitis B during the “window period" of acute HBV infection (ie, after disappearance of hepatitis B surface antigen and prior to appearance of hepatitis B surface antibody).

Performing Laboratory
Mayo Clinic Laboratories, Rochester, Minnesota

Additional Information
Hepatitis B Surface Antigen Screen, Serum

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.