Antinuclear Antibodies (ANA)

Message

Sendout, Mayo test code: ANA2

Test Code

LAB147

Alias/See Also

ANA1
ANA2
Antinuclear Antibody, Serum
Hep-2
ANA (order test ANA2)
ANA (Antinuclear Antibodies)
Antinuclear Antibodies (ANA), HEp-2 substrate, Serum
LE (Lupus Erythematosus) Prep
ANA Screen
ANA

CPT Codes
86038

Preferred Specimen

0.5 mL serum from a gold serum gel tube

Minimum Volume

0.4 mL

Other Acceptable Specimens

Red tube

Instructions

Centrifuge and aliquot serum into a plastic vial.

Transport Container

Plastic vial

Transport Temperature

Refrigerated

Specimen Stability

Refrigerated (preferred): 21 days
Frozen: 21 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis: Reject
Gross lipemia: Reject
Gross icterus: OK

Methodology
Enzyme-Linked Immunosorbent Assay (ELISA)

FDA Status
Approved

Setup Schedule

Monday through Saturday

Report Available

1 day

Limitations

Results for antinuclear antibodies (ANA) performed by enzyme-linked immunosorbent assay (ELISA) and traditional immunofluorescence assay (IFA) in the Mayo Clinic Antibody Immunology Laboratory show both methods to be substantially equivalent for the evaluation of common connective tissue diseases (CTD); systemic lupus erythematosus, Sjogren syndrome, and mixed connective tissue disease.(2) Negative results do not rule out the diagnosis of other CTD, such as systemic sclerosis and inflammatory myopathies, and overlap syndromes associated with complex antigens optimally detected in with the ANA IFA and associated with nucleolar, certain cytoplasmic and/or speckled patterns. For autoimmune hepatitis and juvenile idiopathic arthritis, ANA IFA remains the optimal testing method as the target autoantigens are largely unknown and not included in the ELISA or other solid-phase immunoassays.(3-6)

ANA may also be detectable following viral illnesses, in individuals with chronic infections, or in patients treated with many different medications.

Reference Range

Included with report

Clinical Significance

Evaluating patients at-risk for antinuclear antibodies-associated systemic autoimmune rheumatic disease particularly systemic lupus erythematosus, Sjogren syndrome, and mixed connective tissue disease

Performing Laboratory
Mayo Clinic Laboratories, Rochester, Minnesota

Additional Information
Antinuclear Antibodies (ANA), Serum

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.