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Blood Culture, Pediatric
Test Code1236201048
Alias/See Also
Blood
C BLD
Culture
Culture BLD
C BLD
Culture
Culture BLD
CPT Codes
87040
Preferred Specimen
Whole blood in a Bactec peds blood culture
Instructions
1. Disinfect blood culture bottles by rubbing stoppers with 70% alcohol and allowing to dry.
2. If palpation is needed after site prep a sterile glove must be used.
3. Using ChloraPrep One-Step Kit, pinch the wings on the applicator to break the ampule. Do not touch sponge. Wet the sponge by repeatedly pressing and releasing the sponge against the treatment area until liquid is visible on the skin. Use gentle repeated back-and-forth strokes for 30 seconds with the CHG sponge and allow to air dry for 30 seconds. Do not blot or wipe away. Discard sponge.
4. Perform venipuncture using a vacutainer safety lok butterfly needle. Do NOT repalpate the venipuncture site.
5. Inoculate blood cultures bottles in the following order: aerobic, anaerobic, (if ordered) and any other blood work is collected last.
6. Multiple blood culture sets may be collected at the same time from separate venipuncture sites.
7. Mix bottles.
8. Label samples noting blood culture set number (i.e. set 1, set 2), and site of collection (peripheral or indicate which line).
2. If palpation is needed after site prep a sterile glove must be used.
3. Using ChloraPrep One-Step Kit, pinch the wings on the applicator to break the ampule. Do not touch sponge. Wet the sponge by repeatedly pressing and releasing the sponge against the treatment area until liquid is visible on the skin. Use gentle repeated back-and-forth strokes for 30 seconds with the CHG sponge and allow to air dry for 30 seconds. Do not blot or wipe away. Discard sponge.
4. Perform venipuncture using a vacutainer safety lok butterfly needle. Do NOT repalpate the venipuncture site.
5. Inoculate blood cultures bottles in the following order: aerobic, anaerobic, (if ordered) and any other blood work is collected last.
6. Multiple blood culture sets may be collected at the same time from separate venipuncture sites.
7. Mix bottles.
8. Label samples noting blood culture set number (i.e. set 1, set 2), and site of collection (peripheral or indicate which line).
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 48 hours
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Improper specimen collection, storage, and transport
Specimen refrigerated
Blood culture bottle received cracked or broken
Inadequate quantity
Specimen refrigerated
Blood culture bottle received cracked or broken
Inadequate quantity
Reference Range
No growth
Performing Laboratory
Guthrie Clinic Lab
Corning Hospital Lab
Troy Hospital Lab
GMG Laboratory at Towanda
Cortland Medical Center Lab
