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Antiphospholipid Syndrome Panel
Test Code1235301001 and 1235500538
Alias/See Also
Antiphospholipid Syndrome Panel
Lupus Anticoagulant
Beta 2 Glycoprotein
Lupus Anticoagulant
Beta 2 Glycoprotein
CPT Codes
86147, 86146, 85705
Includes
Cardiolipin (IgA, IgG, IgM)
B2 Glycoprotein (IgA, IgG, IgM)
Lupus Anticogulant
B2 Glycoprotein (IgA, IgG, IgM)
Lupus Anticogulant
Preferred Specimen
1. Serum (1 mL) collected in a gold-top serum separator tube (SST)
2. Plasma collected in a light blue-top 3.2% sodium citrate tube
2. Plasma collected in a light blue-top 3.2% sodium citrate tube
Minimum Volume
0.5 mL
Instructions
1. SST tube: Spin and do not separate. Store and transport refrigerated.
2. Blue Citrate tube: Correct ratio of blood to citrate is critical. Avoid hemolysis. Tube must be filled completely and mixed gently 8 times.
Unopened light blue-top citrate tube: Specimens are kept at room temperature and centrifuged within 4 hours of collection to obtain platelet-poor plasma. Centrifuged specimens must be tested within 4 hours of collection. When testing is delayed more than 4 hours, remove the platelet-poor plasma using a plastic pipette taking care to avoid the WBC/platelet buffy layer and transport platelet-poor plasma into a labeled aliquot tube. Freeze immediately. Frozen specimens are stable 14 days.
Platelet-poor Plasma – Centrifuge time varies - Refer to centrifuge time below:
2500-3500 RPM 15 minutes (most outpatient benchtop centrifuges)
3600 RPM 10 minutes
5100 RPM 5 minutes
5100-7200 RPM 3 minutes (Clinical lab STAT testing)
2. Blue Citrate tube: Correct ratio of blood to citrate is critical. Avoid hemolysis. Tube must be filled completely and mixed gently 8 times.
Unopened light blue-top citrate tube: Specimens are kept at room temperature and centrifuged within 4 hours of collection to obtain platelet-poor plasma. Centrifuged specimens must be tested within 4 hours of collection. When testing is delayed more than 4 hours, remove the platelet-poor plasma using a plastic pipette taking care to avoid the WBC/platelet buffy layer and transport platelet-poor plasma into a labeled aliquot tube. Freeze immediately. Frozen specimens are stable 14 days.
Platelet-poor Plasma – Centrifuge time varies - Refer to centrifuge time below:
2500-3500 RPM 15 minutes (most outpatient benchtop centrifuges)
3600 RPM 10 minutes
5100 RPM 5 minutes
5100-7200 RPM 3 minutes (Clinical lab STAT testing)
Transport Temperature
1. Serum: Refrigerated
2. Plasma: Frozen
2. Plasma: Frozen
Methodology
Phadia
Performing Laboratory
Guthrie Clinic Lab
