| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
BK Virus DNA, Quantitative, Real-Time PCR, Plasma
Test Code11274
CPT Codes
87799
Preferred Specimen
2 mL plasma collected in an EDTA (lavender-top) tube
Minimum Volume
1 mL
Instructions
Do not freeze whole blood or samples stored in primary tube. Freshly drawn specimens (whole blood) may be stored at 2-25° C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation. Follow manufacturer's instructions for collection tube handling.
Transport Container
Transport tube
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 6 days
Frozen -20° C: 6 months
Frozen -70° C: 6 months
Refrigerated: 6 days
Frozen -20° C: 6 months
Frozen -70° C: 6 months
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Sodium heparin (green-top) tube • Lithium heparin (green-top) tube • Unspun PPT tubes • Frozen whole blood
Methodology
Real-Time Polymerase Chain Reaction (PCR)
Setup Schedule
Daily
Reference Range
| BK Virus DNA, QN PCR | Not Detected (IU/mL) |
| BK Virus DNA, QN PCR | Not Detected (Log IU/mL) |
Clinical Significance
This test detects and quantitates BK Virus DNA in plasma specimens of kidney transplant recipients who are at risk of developing BKV-associated nephropathy (BKVAN) and associated hemorrhagic cystitis leading to kidney failure. Prospective viral load monitoring of BKV DNA in plasma (and urine) is useful to exclude a diagnosis of BKVAN and to monitor the course of BKV infection. The assay is calibrated to the First World Health Organization International Standard for BKV DNA with a Reportable Range of 21.5-100,000,000 IU/mL (1.33-8.00 log IU/mL).
Performing Laboratory
| Quest Diagnostics Nichols Institute-Chantilly VA |
| 14225 Newbrook Drive |
| Chantilly, VA 20151-2228 |
