NRAS Mutation Analysis

Test Code

16818

CPT Codes
81311

Preferred Specimen

Formalin-fixed, paraffin-embedded tissue

Minimum Volume

3 mL whole blood • 1 mL bone marrow aspirate • 4 unstained charged (+) slides

Other Acceptable Specimens

5 mL whole blood collected in an EDTA (lavender-top) tube or sodium heparin (green-top) tube • 3 mL bone marrow aspirate submitted in an EDTA (lavender-top) tube or sodium heparin (green-top) tube • 8 unstained charged (+) slides

Instructions

Paraffin block is the preferred sample type. Sample source and block ID are required on the requisition form and a pathology report must be submitted.

Whole blood (acceptable): Follow standard whole blood collections procedures. Collect 3-5 mL whole blood in an EDTA tube. Record sample type, collection time, and date on both tube and requisition form.

Bone marrow (acceptable) collection: The notation of sample type, collection time, and date on the tube and requisition form is required. Ship sample refrigerated or room temperature. Ship immediately to maintain stability.

Do not reject specimens, send to laboratory for screening.

Transport Container

Formalin-fixed, paraffin-embedded tissue block

Transport Temperature

Room temperature

Specimen Stability

Formalin-fixed, paraffin-embedded tissue/slides
Room temperature: 5 years
Refrigerated: 5 years
Frozen: Unacceptable

Whole blood and bone marrow
Room temperature: 72 hours
Refrigerated: 72 hours
Frozen: Unacceptable

Methodology
Next Generation Sequencing

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

a.m. Daily

Reference Range

Not detected

Clinical Significance

NRAS mutations have been described in a variety of cancers including 3-5% of colorectal carcinomas, 10-20% of melanomas and 10% of acute myeloid leukemias. Mutations leading to an amino acid substitution at positions 12, 13, 61 and 146 of NRAS are the most common in naturally occurring neoplasms.

Performing Laboratory

Quest Diagnostics Nichols Institute-Chantilly VA

14225 Newbrook Drive

Chantilly, VA 20151-2228

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.