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Lactose Tolerance Test, 5 Specimens (50g)
Test Code7675
CPT Codes
82951, 82952 (x2)
Includes
Fasting specimen, 1/2-hour, 1-hour, 2-hour, and 3-hour specimens
Preferred Specimen
Five - 1 mL plasma specimens collected in fluoride/oxalate (gray-top) tubes
Patient Preparation
Note: This cannot be performed at a patient service center because of the preparation step required for the lactol solution.
Fasting required. Fasting is defined as no consumption of food or beverage other than water for at least 8 hours before testing.
Minimum Volume
0.5 mL for each specimen
Other Acceptable Specimens
Plasma collected in: Lithium heparin (green-top) tube • Sodium heparin (green-top) tube • Serum (separated from cells immediately) • Spun serum separator tube (SST)
Instructions
Obtain fasting specimen. Immediately after fasting specimen is obtained, have the patient ingest a 50 g dose of lactose (obtained through client supply). Collect subsequent specimens 1/2, 1, 2, and 3 hours, labeling each sample at the time of draw with the specific draw time.
LacTest Preparation instructions: If using LacTest, dissolve two 25 g packets into 6-8 oz of water to make the 50 g dose required. Mix thoroughly. Because of the relative insolubility of lactose, mix frequently during ingestion as necessary. If any lactose precipitate remains in the glass, swirl with a small amount of additional water and drink.
For pediatric use, dissolve 1 gram of LacTest for every 1 kg (2.2 lbs) of the patient's body weight in 6-8 oz of water.
LacTest Preparation instructions: If using LacTest, dissolve two 25 g packets into 6-8 oz of water to make the 50 g dose required. Mix thoroughly. Because of the relative insolubility of lactose, mix frequently during ingestion as necessary. If any lactose precipitate remains in the glass, swirl with a small amount of additional water and drink.
For pediatric use, dissolve 1 gram of LacTest for every 1 kg (2.2 lbs) of the patient's body weight in 6-8 oz of water.
Transport Container
Fluoride/oxalate (gray-top) tubes
Transport Temperature
Room temperature
Specimen Stability
Sodium fluoride plasma
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 28 days
Heparinized plasma
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 28 days
Serum
Room temperature: 4 days
Refrigerated: 7 days
Frozen: 28 days
Whole blood (gray-top) tube
Room temperature: 24 hours
Refrigerated: 72 hours
Frozen: Unacceptable
Note: Serum and plasma submissions must be separated from cells.
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 28 days
Heparinized plasma
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 28 days
Serum
Room temperature: 4 days
Refrigerated: 7 days
Frozen: 28 days
Whole blood (gray-top) tube
Room temperature: 24 hours
Refrigerated: 72 hours
Frozen: Unacceptable
Note: Serum and plasma submissions must be separated from cells.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Unspun serum or plasma separator tube (SST or PST) • Red-top or green-top tube (serum or heparinized plasma not separated from cells) • Anticoagulants other than lithium heparin, sodium heparin or fluoride/oxalate
Methodology
Spectrophotometry (SP)
Setup Schedule
Mon-Sat
Report Available
1 day
Clinical Significance
Results may be used to evaluate deficiency of small bowel mucosal lactase, which may be associated with intolerance to lactose, manifested by diarrhea and other symptoms following ingestion of milk.
Performing Laboratory
| Quest Diagnostics-Pittsburgh Lab |
| 875 Greentree Road |
| Pittsburgh, PA 15220-3508 |
