HIV-1/2 Ab Differentiation (Supplemental Use Only) with Reflex HIV 1 RNA, QL RT PCR

Test Code
93170


CPT Codes
86701, 86702

Includes
If HIV-1/2 Antibody Differentiation is Indeterminate or Negative, then HIV-1 RNA, Qualitative Real-Time PCR will be performed at an additional charge (CPT code(s): 87535).


Preferred Specimen
3 mL serum


Minimum Volume
2 mL


Other Acceptable Specimens
Serum collected in: Red-top tube (no gel) • Plasma collected in: EDTA (lavender-top) tube


Instructions
Serum: Collect in a serum separator tube or red-top tube (no gel). The tube should be centrifuged after clotting. Spun serum separator tubes may be submitted at room temperature or refrigerated unopened without transfer to a plastic, screw-cap vial. Red-top tube (no gel) serum should be transferred to a plastic, screw-cap vial and submitted for testing.

Plasma: Collect in EDTA (lavender top) tube and centrifuge tube within 24 hours of collection. Transfer plasma to a plastic, screw-cap vial, label as plasma, and submit for testing.

Submitted tubes must be labeled with patient identifier and submitted only for HIV testing.


Transport Container
Serum separator tube (SST)


Transport Temperature
Room temperature


Specimen Stability
Room temperature: 72 hours
Refrigerated: 5 days
Frozen: 30 days


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Frozen serum separator tube (SST)


Methodology
Immunoassay (IA) • Real-Time Polymerase Chain Reaction

Setup Schedule
Mon, Thurs


Reference Range
HIV-1 Antibody Negative
HIV-2 Antibody Negative


Performing Laboratory
Quest Diagnostics-Pittsburgh Lab
875 Greentree Road
Pittsburgh, PA 15220-3508




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.