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RARA Break-Apart
MessageProbes: RARA (17q21) Disease(s): APL, AML
Test Code
LAB6167
CPT Codes
88377x1 manual or 88374x1 automated.
Preferred Specimen
Bone marrow aspirate: 1-2 mL sodium heparin tube. EDTA tube is acceptable.
Peripheral blood: 2-5 mL sodium heparin tube. EDTA tube is acceptable.
Fresh, unfixed tissue: Tissue in RPMI.
Fluids: Equal parts RPMI to specimen volume.
Paraffin block or cut slides: Not available.
Peripheral blood: 2-5 mL sodium heparin tube. EDTA tube is acceptable.
Fresh, unfixed tissue: Tissue in RPMI.
Fluids: Equal parts RPMI to specimen volume.
Paraffin block or cut slides: Not available.
Instructions
Refrigerate specimen. Do not freeze. Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.
Methodology
FISH
Report Available
3-5 days
Clinical Significance
This RARA break-apart probe is useful for detecting variant RARA translocations with partners other than PML such as t(5;17) and t(11;17). Testing is recommended when FISH for t(15;17) is negative but extra RARA signals are seen to distinguish variant RARA translocations from trisomy 17. If indicated, follow-up metaphase FISH may be added to identify rearrangement partner chromosomes as an aid to therapy selection. Testing is also useful for verifying RARA status when morphology is suspicious for APL but t(15;17) FISH is negative.
Performing Laboratory
Neogenomics Laboratories
