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BRAF Mutation Analysis by PCR
MessageBi-directional sequencing of exon 15 of the BRAF gene, which includes qualitative detection of V600 mutations E, K, D, and others, plus other significant exon 15 mutations. For solid tumors, tumor enrichment is performed before extraction. Expanded coverage for BRAF exons 11 & 15 is available in the RAS/RAF Panel. Testing is available separately or in combination with HRAS, KRAS, and NRAS in the RAS/RAF Panel. Testing is approved for specimens from the state of New York.
Test Code
LAB6640
Alias/See Also
BRAF V600
CPT Codes
81210x1
Preferred Specimen
•Fine needle aspirate (FNA): Requisition must note specimen is FNA. Fresh cells in suspension, unstained air-dried smears (approx. 6-8 slides), or FFPE cell blocks are acceptable if pathologist attaches note verifying sample has >30% tumor or abnormal cells (required). Minimum 10^6 cells.
•Peripheral blood: 5 mL in EDTA tube.
•Bone marrow: 2 mL in EDTA tube.
•Peripheral blood: 5 mL in EDTA tube.
•Bone marrow: 2 mL in EDTA tube.
Instructions
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. All slides can be packed at room temperature.
Methodology
Molecular
Report Available
7 days
Clinical Significance
Useful in selection of melanoma patients for vemurafenib therapy, for determination of prognosis in thyroid and colon cancers, for predicting response to anti-EGFR therapy in colon cancer, and as aid to diagnosis of hairy cell leukemia.
Performing Laboratory
Neogenomics Laboratories
