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| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
PROLACTIN WITH REFLEX TO MACROPROLACTIN
Test Codelab6055
Alias/See Also
PRL, MACRO
CPT Codes
84146
Includes
Prolactin values that exceed the established threshold of 23.3 ng/mL will reflex a Macroprolactin. The Macroprolactin procedure differentiates between monomeric and oligomeric forms of prolactin in human plasma. Macroprolactin and oligomers are precipitated by using a 25% aqueous polyethylene glycol solution (ratio 1:1). After centrifugation, the supernatant containing monomeric prolactin is used in the Prolactin assay in the same way as native samples. Monomeric prolactin will be expressed as a percentage and reported.
Preferred Specimen
Green Top/Gel LiHep
Minimum Volume
2.0 mL
Methodology
ECLIA
Setup Schedule
Set Up:Daily Report Available:1 day
Reference Range
Prolactin:
If Prolactin >23.3 ng/mL:
Total prolactin level in this serum is elevated due the possibility of the presence of macroprolactin. Prolactin, monomeric recovery was determined by PEG precipitation.
The percentage recovery of prolactin following precipitation reported:
1) Recovery >60%: Sample contains a significant amount of monomeric prolactin and is consistent with hyperprolactinemia.
2) Recovery between 40 and 60%: Sample may contain macroprolactin and/or oligomeric prolactin in addition to monomeric prolactin.
3) Recovery <40%: Sample contains a significant amount of macroprolactin and/or oligomeric prolactin in addition to monomeric prolactin.
| Males: 4.0 - 15.2 ng/mL Females: 4.8 - 23.3 ng/mL |
If Prolactin >23.3 ng/mL:
Total prolactin level in this serum is elevated due the possibility of the presence of macroprolactin. Prolactin, monomeric recovery was determined by PEG precipitation.
The percentage recovery of prolactin following precipitation reported:
1) Recovery >60%: Sample contains a significant amount of monomeric prolactin and is consistent with hyperprolactinemia.
2) Recovery between 40 and 60%: Sample may contain macroprolactin and/or oligomeric prolactin in addition to monomeric prolactin.
3) Recovery <40%: Sample contains a significant amount of macroprolactin and/or oligomeric prolactin in addition to monomeric prolactin.
Clinical Significance
Prolactin is produced by the anterior pituitary and its secretion is regulated physiologically by inhibitory and releasing factors of the hypothalamus. Prolactin appears in the blood promptly after administration of thyrotropin-releasing hormone (TRH). The major physiologic action of prolactin is the initiation and maintenance of lactation in women. Hyperprolactinemia has been established as a common cause of infertility and gonadal disorders in men and women. Prolactin has been shown to inhibit the secretion of ovarian steroids and to interfere with follicle maturation and the secretion of LH and FSH in the human female. Measurement of elevated serum prolactin levels may provide the first quantitative evidence of pituitary dysfunction. Quantitation of prolactin levels is also of interest in the evaluation and management of patients with amenorrhea and galactorrhea. Various factors other than disease states have been found to influence the prolactin levels. Factors which increase prolactin concentrations include: pregnancy, breast stimulation, stress, coitus, administration of estrogens, progesterone, androgens, some psychotropic and antihypertensive drugs, and TRH. Factors which decrease prolactin concentrations include the administration of L-dopa and bromocriptine.
Performing Laboratory
GBMC Chemistry
