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AFB CULTURE & STAIN
Test CodeAlias/See Also
ACID-FAST BACILLI CULTURE/SMEAR
CPT Codes
87116, 87206
Preferred Specimen
Collect in sterile container. Early morning specimen preferred
Minimum Volume
Instructions
The specimen should be submitted to the lab as soon as possible after collection, preferably in less than 1 hour. All specimen requests for acid-fast (AFB) culture automatically receive an acid-fast smear. If mycobacteria are isolated, isolates will be referred to the Maryland State Health Department for definitive identification. Sputum, expectorated or induced, is the principal specimen obtained for the diagnosis of pulmonary tuberculosis. FOR OPTIMAL ISOLATION OF THE ORGANISM FROM SPUTUM IN PATIENTS SUSPECTED OF HAVING PULMONARY TUBERCULOSIS, IT IS RECOMMENDED THAT THREE SAMPLES SHOULD BE COLLECTED AT 8-24 HOUR INTERVALS (24 HOURS WHEN POSSIBLE) AND SHOULD INCLUDE AT LEAST ONE FIRST MORNING SPECIMEN.
Transport Container
sterile cup
sterile swab
Setup Schedule
Report Available
Final report in 6 weeks
Reference Range
No growth of acid-fast bacilli after 6 weeks incubation
Performing Laboratory
GBMC Microbiology