HPV mRNA E6-E7rfx 16, 18-45

Test Code

LAB5184

Quest Code

90942X

CPT Codes
87624

Includes

If HPV mRNA E6/E7 is detected, HPV Genotypes 16, 18/45 will be performed at an additional charge (CPT Code(s): 87625).

Preferred Specimen

5 mL Liquid Cytology (PreservCyt(R)) Preservative
(ThinPrep(R))

Minimum Volume

1 mL

Specimen Stability

Room temperature: 30 days
Refrigerated: 90 days
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Cervical swabs in Digene(R) HC Cervical Sampler,
Digene(R)vials, Vaginal swabs, SurePath(R) vials,
Received frozen

Methodology
Transcription-Mediated Amplification (TMA)

Setup Schedule

Set up: Mon-Fri a.m.; Report available: 3 days

Reference Range

HPV mRNA E6/E7

Not detected

This test was performed using the APTIMA® HPV Assay (Gen-Probe Inc.).

This assay detects E6/E7 viral messenger RNA (mRNA) from 14 high-risk HPV types (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68).

Clinical Significance

The presence of E6/E7 messenger RNA from 14 high risk HPV types indicates incorporation of HPV DNA into the host cells. Proteins expressed from E6-E7 polycistronic mRNA alter cellular p53 and retinoblastoma protein functions, leading to disruption of cell-cycle check points and cell genome instability. This information, together with the physicians assessment of cytology history, other risk factors, and professional guidelines, may be used to guide patient management.
This assay detects E6/E7 viral messenger RNA (mRNA) from 14 high-risk HPV types (16,18,31,33,35,39,45,51,52,56,58, 59,66,68).

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.