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HPV mRNA E6/E7
Test CodeLAB5183
Quest Code
90887X
Alias/See Also
QB.90887X
CPT Codes
87624
Preferred Specimen
3 mL Residual (PreverCyt(R)) fluid
1. Collect cervical specimens in ThinPrep (R) Pap Test
vials containing PreservCyt (R) Solution with broom-type
or cytobrush/spatula collection devices, according to
the manufacturer's instructions.
2. Alternatively, 1 mL PreservCyt(R) can be aliquoted
pre-cytology or post-cytology.
1. Collect cervical specimens in ThinPrep (R) Pap Test
vials containing PreservCyt (R) Solution with broom-type
or cytobrush/spatula collection devices, according to
the manufacturer's instructions.
2. Alternatively, 1 mL PreservCyt(R) can be aliquoted
pre-cytology or post-cytology.
Minimum Volume
1.5 mL
Specimen Stability
Room temperature: 30 days
Refrigerated: 90 days
Frozen: Unacceptable
Refrigerated: 90 days
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
The following specimens are unacceptable: Cervical
swabs in Digene HC Cervical Sampler, Digene vials,
Vaginal swabs, and SurePath vials.
swabs in Digene HC Cervical Sampler, Digene vials,
Vaginal swabs, and SurePath vials.
Methodology
Transcription-Mediated Amplification (TMA)
Setup Schedule
Set up: Mon-Fri a.m.; Report availavle: 3 days
Reference Range
Not detected
Clinical Significance
The presence of E6/E7 messenger RNA from 14 high risk HPV types indicates incorporation of HPV DNA into the host cells. Proteins expressed from E6-E7 polycistronic mRNA alter cellular p53 and retinoblastoma protein functions, leading to disruption of cell-cycle check points and cell genome instability. This information, together with the physician’s assessment of cytology history, other risk factors, and professional guidelines, may be used to guide patient management.
