|
|
| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
STREP GP B DNA,PCR,W-RFL
Test Code91770X
CPT Codes
PROF
Includes
If Streptococcus Group B DNA, PCR is Detected, Susceptibility, Aerobic Bacteria, MIC will be performed at an additional charge (CPT code(s): 87186 or 87184).
Preferred Specimen
Preferred:
Vaginal/Rectal swab in Amies gel (blue-cap) transport
medium or Amies liquid (red-cap) transport medium
Acceptable:
Vaginal/Rectal swab in Liquid Stuart transport medium
OR
2 mL LIM broth tube (pre-incubated)
No other specimens are acceptable
Instructions:
In order to obtain an adequate specimen, the procedure
for specimen collection must be followed closely. Using
recommended swab, vaginal-rectal specimens are
collected according to the following procedure:
1. Wipe away excessive amount of secretion or discharge
from the vaginal area.
2. Carefully insert the swab into the lower one-third
part of vagina, and sample secretions from the mucosa.
3. Carefully insert the same swab, approximately 2.5 cm
beyond the anal sphincter, and gently rotate to sample
anal crypts.
4. Replace the swab in its container.
If submitting broth, inoculate LIM Broth with vaginal/
rectal swab and incubate at 35-37 C for 18-24 hours.
Note: Endocervical, perianal, perineal and perirectal
are not appropriate samples for this test. Do not use a
speculum for collection
Vaginal/Rectal swab in Amies gel (blue-cap) transport
medium or Amies liquid (red-cap) transport medium
Acceptable:
Vaginal/Rectal swab in Liquid Stuart transport medium
OR
2 mL LIM broth tube (pre-incubated)
No other specimens are acceptable
Instructions:
In order to obtain an adequate specimen, the procedure
for specimen collection must be followed closely. Using
recommended swab, vaginal-rectal specimens are
collected according to the following procedure:
1. Wipe away excessive amount of secretion or discharge
from the vaginal area.
2. Carefully insert the swab into the lower one-third
part of vagina, and sample secretions from the mucosa.
3. Carefully insert the same swab, approximately 2.5 cm
beyond the anal sphincter, and gently rotate to sample
anal crypts.
4. Replace the swab in its container.
If submitting broth, inoculate LIM Broth with vaginal/
rectal swab and incubate at 35-37 C for 18-24 hours.
Note: Endocervical, perianal, perineal and perirectal
are not appropriate samples for this test. Do not use a
speculum for collection
Specimen Stability
Vaginal/Rectal swab:
Room temperature: 24 hours
Refrigerated: 6 days
Frozen: Unacceptable
LIM broth tube:
Room temperature: Unacceptable
Refrigerated: 72 hours
Frozen: Unacceptable
Room temperature: 24 hours
Refrigerated: 6 days
Frozen: Unacceptable
LIM broth tube:
Room temperature: Unacceptable
Refrigerated: 72 hours
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimens in transport media other than those listed;
received frozen; LIM broth received at room temperature
received frozen; LIM broth received at room temperature
Methodology
Culture Enhanced • Polymerase Chain Reaction (PCR)
Setup Schedule
Set up: Sun-Fri; Report available: 3 days for negatives
Additional 3 days if suceptibility performed
Additional 3 days if suceptibility performed
Reference Range
Not detected
Clinical Significance
Detect Group B Streptococcus (GBS) vaginal/rectal colonization to prevent GBS sepsis and other GBS infections in newborns.
Performing Laboratory
Quest Diagnostics HORSHAM
