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CATECHOLAMINES,FRAC-TOT,PLAS
Test Code314X
CPT Codes
82384
Includes
Dopamine, Epinephrine, Norepinephrine and Total Catecholamines (calculated)
Preferred Specimen
4 mL plasma collected in a green-top (sodium heparin)
tube
Draw specimen in a pre-chilled green-top vacutainer.
INSTRUCTIONS
After drawing specimen, place green-top tube in ice
bath. Centrifuge for 20 Minutes 1000XG.
The plasma **MUST** be free of RBC'S.
Freeze on dry ice to transport.
Patients should be relaxed in either a supine or
upright position before blood is drawn. States of
anxiety and stress can cause fluctuations in the
catecholamine levels.
Patient should avoid alcohol, coffee, tea, tobacco
and strenuous exercise prior to collection.
Overnight fasting is preferred.
Transport temperature: Frozen only, do not thaw
Other acceptable specimens:
GN, green-top tube, lithium heparin) or
(T, tan-top tube, sodium heparin, lead-free)
tube
Draw specimen in a pre-chilled green-top vacutainer.
INSTRUCTIONS
After drawing specimen, place green-top tube in ice
bath. Centrifuge for 20 Minutes 1000XG.
The plasma **MUST** be free of RBC'S.
Freeze on dry ice to transport.
Patients should be relaxed in either a supine or
upright position before blood is drawn. States of
anxiety and stress can cause fluctuations in the
catecholamine levels.
Patient should avoid alcohol, coffee, tea, tobacco
and strenuous exercise prior to collection.
Overnight fasting is preferred.
Transport temperature: Frozen only, do not thaw
Other acceptable specimens:
GN, green-top tube, lithium heparin) or
(T, tan-top tube, sodium heparin, lead-free)
Minimum Volume
2.5 mL Frozen Plasma
Specimen Stability
Room temperature: 6 hours
Refrigerated: 6 hours
Frozen: 30 days
Refrigerated: 6 hours
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Excessive hemolysis.
Patient receiving isoproterenol or isoetharine
Specimens received not frozen.
Patient receiving isoproterenol or isoetharine
Specimens received not frozen.
Methodology
High Performance Liquid Chromatography (HPLC) • Electrochemical Detection
Setup Schedule
Set up: Mon,Wed,Fri a.m.; Report available: 3 days
Limitations
Recent surgery, traumatic injury, upright posture, cold, anxiety, pain, clonidine withdrawal, and concurrent acute or chronic illness may produce elevated results.
Reference Range
Adult reference ranges:
Supine Upright
Total: 123-671 pg/mL 242-1125 pg/mL
Epinephrine: <50 pg/mL <95 pg/mL
Norepinephrine: 112-658 pg/mL 217-1109 pg/mL
Dopamine: <30 pg/mL <30 pg/mL
Pediatric range:(Upright values are not available)
Epinephrine: 3-15 years: supine: 464 pg/mL or Less
Norepinephrine: 3-15 years: supine: 1251 pg/mL or Less
Dopamine: 3-15 years: supine: Less than 60 pg/mL
Due to stress, plasma catecholamine levels are generally
unreliable in infants and small children. Urinary
catecholamine assays are more reliable.
Supine Upright
Total: 123-671 pg/mL 242-1125 pg/mL
Epinephrine: <50 pg/mL <95 pg/mL
Norepinephrine: 112-658 pg/mL 217-1109 pg/mL
Dopamine: <30 pg/mL <30 pg/mL
Pediatric range:(Upright values are not available)
Epinephrine: 3-15 years: supine: 464 pg/mL or Less
Norepinephrine: 3-15 years: supine: 1251 pg/mL or Less
Dopamine: 3-15 years: supine: Less than 60 pg/mL
Due to stress, plasma catecholamine levels are generally
unreliable in infants and small children. Urinary
catecholamine assays are more reliable.
Clinical Significance
The three catecholamines (norepinephrine, epinephrine,
and dopamine) are the principal secretory products of
neural tissue. Clinically, the measurement of
circulating catecholamines is valuable in the diagnosis
of catecholamine secreting tumors associated chiefly
with hypertension (pheochromocytomas,
neuroblastomas, and gangliomas) and with the
evaluation of orthostatic hypotension.
and dopamine) are the principal secretory products of
neural tissue. Clinically, the measurement of
circulating catecholamines is valuable in the diagnosis
of catecholamine secreting tumors associated chiefly
with hypertension (pheochromocytomas,
neuroblastomas, and gangliomas) and with the
evaluation of orthostatic hypotension.
Performing Laboratory
Quest Diagnostics Nichols Chantilly
