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Cytomegalovirus DNA, Qualitative, Real-Time PCR
Test CodePreferred Specimen
Plasma collected in an EDTA (lavender-top), ACD B (yellow-top), or PPT Potassium EDTA (white-top) tube OR
Serum, CSF, Bronchoalveolar lavage, Eye fluids, Amniotic fluid, Amniotic fluid supernatant collected in a sterile leak-proof container OR random urine collected in a sterile screw cap container
Minimum Volume
Other Acceptable Specimens
Instructions
Blood collected in tubes with heparin anticoagulant are not accepted for this test.
Plasma: Collect blood in sterile tubes containing EDTA or ACD as anticoagulant or in Plasma Preparation Tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw-capped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.
Whole blood: Collect whole blood in sterile tubes containing EDTA or ACD as anticoagulant. Store refrigerated. Do not freeze whole blood.
CSF, amniotic fluid, urine and tissue: Collect in a sterile container and store refrigerated or frozen.
Transport Temperature
Specimen Stability
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
All other sample types
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Clinical Significance