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Steroid Panel, Congenital Adrenal Hyperplasia (CAH)
Test CodeSPCAH
Includes
Androstenedione
11-Deoxycortisol
Cortisol
DHEA, Unconjugated
17-Hydroxypregnenolone
Progesterone
17-Hydroxyprogesterone
Testosterone, Total, LC/MS/MS
Deoxycorticosterone
11-Deoxycortisol
Cortisol
DHEA, Unconjugated
17-Hydroxypregnenolone
Progesterone
17-Hydroxyprogesterone
Testosterone, Total, LC/MS/MS
Deoxycorticosterone
Preferred Specimen
0.5 mL serum collected in a red-top tube (no gel)
Minimum Volume
0.25 mL
Instructions
Collect specimen in a no additive red-top tube. Spin down immediately and pour off into a plastic transport tube.
Transport Temperature
Frozen
Specimen Stability
Room temperature: 6 hours
Refrigerated: 72 hours
Frozen: 28 days
Refrigerated: 72 hours
Frozen: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Moderate hemolysis • Grossly lipemic • Grossly icteric • Serum separator tube (SST) • Plasma
Methodology
Chromatography/Mass Spectrometry
Setup Schedule
Night
Clinical Significance
This panel is useful for the diagnosis/management of patients with the most common forms (21-hydroxylase or 11-hydroxylase deficiency) of congenital adrenal hyperplasia.