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Cholesterol Panel, Friedewald
Test Code94712
CPT Codes
**Non-orderable Internal Code Only**
Preferred Specimen
1 mL serum collected in a serum separator tube (SST)
Patient Preparation
If a cholesterol measurement is to be performed along with triglycerides, the patient should be fasting 9-12 hours prior to collection. The assay manufacturer Beckman Coulter advises: "N-Acetyl Cysteine (NAC), when administered in therapeutic concentrations (for the treatment of acetaminophen overdose), has been... determined to interfere with assays for... cholesterol, uric acid" where "NAC interference may lead to falsely low results." According to Beckman Coulter, the NAC interference should be insignificant by 12 hours after completion of the initial loading dose of an IV infusion treatment regimen consisting of an initial loading dose of 150 mg/kg administered over 1 hour, a second dose of 50 mg/kg administered over 4 hours and a third dose of 100 mg/kg administered over 16 hours.
Minimum Volume
0.5 mL
Other Acceptable Specimens
Plasma collected in a sodium or lithium (green-top) tube
Instructions
Centrifuge within 30-60 minutes following collection.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 48 hours
Refrigerated: 48 hours
Frozen: 15 days
Refrigerated: 48 hours
Frozen: 15 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis; Moderate to gross icterus; Anticoagulants other than heparin
Methodology
Spectrophotometric
Setup Schedule
Set up: Daily; Report available: Next day
Reference Range
See Laboratory Report